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学科主题: 临床医学
题名:
Direct protective effect of interleukin-10 on articular chondrocytes in vitro
作者: Wang, YQ; Lou, SQ
关键词: interleukin-10 ; chondrocytes ; cell culture ; nitric oxide synthase ; matric metalloprotainase
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-07-01
卷: 114, 期:7, 页:723-725
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: IN-VITRO ; CELLS ; MACROPHAGES ; VIVO
英文摘要:

Objective To assess whether interleukin-10 (IL-10) is chondroprotective in vitro.

Methods Chondrocytes were isolated from femoral cartilage of rats (7 - 10 days) by digestion with collagenase II. The first passage cells were grown in 24- well plates with DMEM, supplemented with 10% fetal bovine serum, for 2 - 4 days. The cells were then cultured in 0.1% fetal bovine serum DMEM medium. and given respectively interleukin-1 (IL-1) 100 mu /ml, IL-1 100 mu /ml + recombinant murine interleukin-10 (mIL-10) 20 ng/ml, rmIL-10 20 ng/ml, and cultured for 48 hours. Scanning electron morphology and immunohistochemical study of nitric oxide synthase 2 and matric metalloproteinase 3 mRNA in situ hybridization were performed. Cell proliferation and morphology were observed under inverted microscope from the beginning of cell culture for three weeks.

Results IL-1 stimulated granule production in the cytoplasma of chondrocytes, and the cells died in the second and third weeks of culture. IL-10 antagonized IL-1, protected the cells from death and maintained chondrocyte proliferation. Scanning electron morphology showed that IL-1 stimulated the formation of numerous microvilli on the cell surface, while thin and less numerous microvilli were found in cultures with IL-10. Immunohistochemical study and in situ hybridization showed that IL-10 inhibited NOS2 and MMP3 expression.

Conclusion IL-10 not only inhibits the synthesis of inflammatory cytokines, but also directly protects chondrocytes by antagonizing IL-1.

语种: 英语
WOS记录号: WOS:000170838300013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58088
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: Beijing Univ, Hosp 3, Dept Orthopaed Surg, Beijing 100083, Peoples R China

Recommended Citation:
Wang, YQ,Lou, SQ. Direct protective effect of interleukin-10 on articular chondrocytes in vitro[J]. CHINESE MEDICAL JOURNAL,2001,114(7):723-725.
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