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Direct protective effect of interleukin-10 on articular chondrocytes in vitro
Wang, YQ; Lou, SQ
关键词Interleukin-10 Chondrocytes Cell Culture Nitric Oxide Synthase Matric Metalloprotainase
刊名CHINESE MEDICAL JOURNAL
2001-07-01
114期:7页:723-725
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]IN-VITRO ; CELLS ; MACROPHAGES ; VIVO
英文摘要

Objective To assess whether interleukin-10 (IL-10) is chondroprotective in vitro.

Methods Chondrocytes were isolated from femoral cartilage of rats (7 - 10 days) by digestion with collagenase II. The first passage cells were grown in 24- well plates with DMEM, supplemented with 10% fetal bovine serum, for 2 - 4 days. The cells were then cultured in 0.1% fetal bovine serum DMEM medium. and given respectively interleukin-1 (IL-1) 100 mu /ml, IL-1 100 mu /ml + recombinant murine interleukin-10 (mIL-10) 20 ng/ml, rmIL-10 20 ng/ml, and cultured for 48 hours. Scanning electron morphology and immunohistochemical study of nitric oxide synthase 2 and matric metalloproteinase 3 mRNA in situ hybridization were performed. Cell proliferation and morphology were observed under inverted microscope from the beginning of cell culture for three weeks.

Results IL-1 stimulated granule production in the cytoplasma of chondrocytes, and the cells died in the second and third weeks of culture. IL-10 antagonized IL-1, protected the cells from death and maintained chondrocyte proliferation. Scanning electron morphology showed that IL-1 stimulated the formation of numerous microvilli on the cell surface, while thin and less numerous microvilli were found in cultures with IL-10. Immunohistochemical study and in situ hybridization showed that IL-10 inhibited NOS2 and MMP3 expression.

Conclusion IL-10 not only inhibits the synthesis of inflammatory cytokines, but also directly protects chondrocytes by antagonizing IL-1.

语种英语
WOS记录号WOS:000170838300013
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58088
专题北京大学第三临床医学院_骨科
作者单位Beijing Univ, Hosp 3, Dept Orthopaed Surg, Beijing 100083, Peoples R China
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Wang, YQ,Lou, SQ. Direct protective effect of interleukin-10 on articular chondrocytes in vitro[J]. CHINESE MEDICAL JOURNAL,2001,114(7):723-725.
APA Wang, YQ,&Lou, SQ.(2001).Direct protective effect of interleukin-10 on articular chondrocytes in vitro.CHINESE MEDICAL JOURNAL,114(7),723-725.
MLA Wang, YQ,et al."Direct protective effect of interleukin-10 on articular chondrocytes in vitro".CHINESE MEDICAL JOURNAL 114.7(2001):723-725.
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