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学科主题临床医学
Endogenous Hydrogen Sulfide Regulates Pulmonary Artery Collagen Remodeling in Rats with High Pulmonary Blood Flow
Li, Xiaohui1,2; Jin, Hongfang1,2; Bin, Geng3; Wang, Li4; Tang, Chaoshu3; Du, Junbao1,2
关键词Hydrogen Sulfide Collagen Pulmonary Artery Inhibitor Of Metalloproteinase-1 (Timp-1) Matrix Metalloproteinase-13 (Mmp-13)
刊名EXPERIMENTAL BIOLOGY AND MEDICINE
2009-05-01
DOI10.3181/0807-RM-230
234期:5页:504-512
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
资助者Natural Science Foundation of Beijing. P. R. China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing. P. R. China ; National Natural Science Foundation of China
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]PROPARGYLGLYCINE ; INACTIVATION ; HYPERTENSION ; PATHOGENESIS ; FIBROBLASTS ; H2S
英文摘要

The mechanisms responsible for the structural remodeling of pulmonary vasculature induced by increased pulmonary blood flow are not fully understood. This study explores the effect of endogenous hydrogen sulfide (H(2)S), a novel gasotransmitter, on collagen remodeling of the pulmonary artery in rats with high pulmonary blood flow. Thirty-two Sprague-Dawley rats were randomly divided into sham, shunt, sham+PPG (D,L-propargylglycine, an inhibitor of cystathionine-gamma-lyase), and shunt+PPG groups. After 4 weeks of shunting, the relative medial thickness (RMT) of pulmonary arteries and H(2)S concentration in lung tissues were investigated. Collagen I and collagen III were evaluated by hydroxyproline assay, sirius-red staining, and immunohistochemistry. Pulmonary artery matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and connective tissue growth factor (CTGF) were evaluated by immunohistochemistry. After 4 weeks of aortocaval shunting, resulting in an elevation of lung tissue H(2)S to 116.4%, rats exhibited collagen remodeling and increased CTGF expression in the pulmonary arteries. Compared with those of the shunt group, lung tissue H(2)S production was lowered by 23.4%, RMT of the pulmonary artery further increased by 39.5%, pulmonary artery collagen accumulation became obvious, and pulmonary artery CTGF expression elevated (P < 0.01) in the shunted rats treated with PPG. However, pulmonary artery MMP-13 and TIMP-1 expressions decreased significantly in rats of shunt+PPG group (P < 0.01). This study suggests that endogenous H(2)S exerts an important regulatory effect on pulmonary collagen remodeling induced by high pulmonary blood flow. Exp Biol Med 234:504-512, 2009

语种英语
所属项目编号7072087 ; 30425010 ; 30821001 ; 30801251 ; 30630031 ; 30872787
资助者Natural Science Foundation of Beijing. P. R. China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing. P. R. China ; National Natural Science Foundation of China
WOS记录号WOS:000265626800004
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58092
Collection北京大学第一临床医学院_儿科
作者单位1.Minist Educ, Kev Lab Mol Cardiovasc Sci, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100034, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Sci Res, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiaohui,Jin, Hongfang,Bin, Geng,et al. Endogenous Hydrogen Sulfide Regulates Pulmonary Artery Collagen Remodeling in Rats with High Pulmonary Blood Flow[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2009,234(5):504-512.
APA Li, Xiaohui,Jin, Hongfang,Bin, Geng,Wang, Li,Tang, Chaoshu,&Du, Junbao.(2009).Endogenous Hydrogen Sulfide Regulates Pulmonary Artery Collagen Remodeling in Rats with High Pulmonary Blood Flow.EXPERIMENTAL BIOLOGY AND MEDICINE,234(5),504-512.
MLA Li, Xiaohui,et al."Endogenous Hydrogen Sulfide Regulates Pulmonary Artery Collagen Remodeling in Rats with High Pulmonary Blood Flow".EXPERIMENTAL BIOLOGY AND MEDICINE 234.5(2009):504-512.
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