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Expression of truncated dystrophin cDNAs mediated by a lentiviral vector
Sun Shunchang1; Chen Haitao1; Chen Weidong2; He Jingbo1; Peng Yunsheng1
关键词Duchenne Muscular Dystrophy Dystrophin Gene Transfer Lentivirus Vector
刊名NEUROLOGY INDIA
2008
56期:1页:52-56
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]DUCHENNE MUSCULAR-DYSTROPHY ; IMMUNODEFICIENCY VIRUS VECTORS ; EFFICIENT TRANSDUCTION ; NONDIVIDING CELLS ; GENE ; TRANSGENE ; PROTEIN ; MUSCLE ; MODEL ; MICE
英文摘要

Background: The success of Duchenne muscular dystrophy gene therapy requires promising tools for gene delivery and mini-gene cassettes that can express therapeutic levels of a functional protein. Aims: To explore the expression feasibility of truncated dystrophin cDNAs mediated by a lentiviral vector derived from feline immunodeficiency virus. Materials and Methods: Three truncated dystrophin cDNAs were constructed by PCR cloning, then these cDNAs were inserted into lentiviral vectors. Recombinant lentiviruses were generated by transient transfection of lentiviral vector constructs into 293Ad(5+) cells. Cultured myoblasts were then infected with recombinant lentiviruses. Expression of truncated dystrophin cDNAs was detected by Western blot analysis. Results: Mediated by lentiviral vectors, three cDNAs constructed by PCR cloning expressed relative truncated dystrophins in cultured myoblasts. Conclusions: Truncated dystrophin cDNAs can express themselves successfully mediated by feline immunodeficiency virus vectors. It offers the possibility of an approach utilizing truncated dystrophin cDNAs and lentiviral vectors toward gene therapy of Duchenne muscular dystrophy.

语种英语
WOS记录号WOS:000254671200011
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58095
专题北京大学深圳医院
作者单位1.So Med Univ, Shenzhen Baoan Hosp, Dept Med Lab Sci, Shenzhen 518101, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China
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GB/T 7714
Sun Shunchang,Chen Haitao,Chen Weidong,et al. Expression of truncated dystrophin cDNAs mediated by a lentiviral vector[J]. NEUROLOGY INDIA,2008,56(1):52-56.
APA Sun Shunchang,Chen Haitao,Chen Weidong,He Jingbo,&Peng Yunsheng.(2008).Expression of truncated dystrophin cDNAs mediated by a lentiviral vector.NEUROLOGY INDIA,56(1),52-56.
MLA Sun Shunchang,et al."Expression of truncated dystrophin cDNAs mediated by a lentiviral vector".NEUROLOGY INDIA 56.1(2008):52-56.
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