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学科主题: 临床医学
题名:
Expression of truncated dystrophin cDNAs mediated by a lentiviral vector
作者: Sun Shunchang1; Chen Haitao1; Chen Weidong2; He Jingbo1; Peng Yunsheng1
关键词: Duchenne muscular dystrophy ; dystrophin ; gene transfer ; lentivirus ; vector
刊名: NEUROLOGY INDIA
发表日期: 2008
卷: 56, 期:1, 页:52-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: DUCHENNE MUSCULAR-DYSTROPHY ; IMMUNODEFICIENCY VIRUS VECTORS ; EFFICIENT TRANSDUCTION ; NONDIVIDING CELLS ; GENE ; TRANSGENE ; PROTEIN ; MUSCLE ; MODEL ; MICE
英文摘要:

Background: The success of Duchenne muscular dystrophy gene therapy requires promising tools for gene delivery and mini-gene cassettes that can express therapeutic levels of a functional protein. Aims: To explore the expression feasibility of truncated dystrophin cDNAs mediated by a lentiviral vector derived from feline immunodeficiency virus. Materials and Methods: Three truncated dystrophin cDNAs were constructed by PCR cloning, then these cDNAs were inserted into lentiviral vectors. Recombinant lentiviruses were generated by transient transfection of lentiviral vector constructs into 293Ad(5+) cells. Cultured myoblasts were then infected with recombinant lentiviruses. Expression of truncated dystrophin cDNAs was detected by Western blot analysis. Results: Mediated by lentiviral vectors, three cDNAs constructed by PCR cloning expressed relative truncated dystrophins in cultured myoblasts. Conclusions: Truncated dystrophin cDNAs can express themselves successfully mediated by feline immunodeficiency virus vectors. It offers the possibility of an approach utilizing truncated dystrophin cDNAs and lentiviral vectors toward gene therapy of Duchenne muscular dystrophy.

语种: 英语
WOS记录号: WOS:000254671200011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58095
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.So Med Univ, Shenzhen Baoan Hosp, Dept Med Lab Sci, Shenzhen 518101, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China

Recommended Citation:
Sun Shunchang,Chen Haitao,Chen Weidong,et al. Expression of truncated dystrophin cDNAs mediated by a lentiviral vector[J]. NEUROLOGY INDIA,2008,56(1):52-56.
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