北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科  > 期刊论文
学科主题: 临床医学
题名:
Long noncoding ribonucleic acid specific for distant metastasis of gastric cancer is associated with TRIM16 expression and facilitates tumor cell invasion in vitro
作者: Yan, Yichao; Shen, Zhanlong1,2; Gao, Zhidong; Cao, Jian; Yang, Yang; Wang, Bo; Shen, Chao; Mao, Shuqiang; Jiang, Kewei; Ye, Yingjiang1,2; Wang, Shan1,2
关键词: gastric cancer ; invasion ; LOC100507069 ; long noncoding RNA ; metastasis
刊名: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
发表日期: 2015-09-01
DOI: 10.1111/jgh.12976
卷: 30, 期:9, 页:1367-1375
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: POOR-PROGNOSIS ; GENE ONTOLOGY ; RNA ; CARCINOGENESIS ; PROLIFERATION ; ORGANIZATION ; PROFILES ; BIOLOGY ; GROWTH
英文摘要:

Background and AimIncreasing evidence has indicated that long noncoding ribonucleic acids (lncRNAs) play a major role in cancers. Although certain lncRNAs has been reported to play a role in gastric cancer (GC), specific lncRNAs involved in distant metastasis of GC remain unknown.

MethodsDifferentially expressed mRNAs and lncRNAs between stage IV and non-stage IV GC were obtained by microarray. Gene ontology and pathway analysis were used to study functions of differential mRNAs. Algorithms were used to predict potential gene targets of cis or trans-acting lncRNAs. Network analysis was performed to analyze each pair of gene-lncRNA, gene-gene, or lncRNA-lncRNA interactions. Expression of lncRNA special for distant metastasis of GC (SDMGC) and target gene TRIM16 were tested in GC tissues and cell lines. RNAi and overexpression were used to observe the biological functions of SDMGC and TRIM16 on GC cells.

Results502 mRNAs and 440 lncRNAs were found to be differentially expressed. 74 gene ontology terms and 38 pathways were associated with the dysregulated transcripts. Fourteen core factors were determined by network analysis. Expression of SDMGC and TRIM16 was upregulated in the distant metastasis tissues, compared with primary GC tissues, which were positive correlation. Silencing of SDMGC or TRIM16 was demonstrated to decrease cell invasion and migration, while upregulated of SDMGC or TRIM16 could promote cell invasion and migration. However, little effect on proliferation, cell cycle, colony formation, and apoptosis was found.

ConclusionsSDMGC is obviously upregulated in stage IV GC and may represent a new marker and therapeutic target for GC treatment.

语种: 英语
所属项目编号: 81372290 ; 81372291 ; 20130001120064 ; RDB2012-19 ; RDB 2013-15
项目资助者: National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Peking University People&prime ; s Hospital Research and Development Funds of China
WOS记录号: WOS:000358685000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58121
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Surg Oncol, Beijing 100044, Peoples R China

Recommended Citation:
Yan, Yichao,Shen, Zhanlong,Gao, Zhidong,et al. Long noncoding ribonucleic acid specific for distant metastasis of gastric cancer is associated with TRIM16 expression and facilitates tumor cell invasion in vitro[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2015,30(9):1367-1375.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yan, Yichao]'s Articles
[Shen, Zhanlong]'s Articles
[Gao, Zhidong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yan, Yichao]‘s Articles
[Shen, Zhanlong]‘s Articles
[Gao, Zhidong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace