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学科主题基础医学
Large quantity of ribosomal RNA exists extracellularly in mouse spleen
Zhao, WP; Liu, XG; Wang, W; Gao, XM
刊名HISTOCHEMICAL JOURNAL
2002-08-01
34期:8-9页:391-396
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]SYSTEMIC LUPUS-ERYTHEMATOSUS ; APOPTOTIC CELLS ; FLOW-CYTOMETRY ; LYMPHOCYTES ; ANTIBODIES
英文摘要

When BALB/c mouse spleens were gently homogenized in saline, the resultant supernatant ( without cells and tissue debris) contained significant amount of 28S and 18S ribosomal RNA, reaching up to 70% of the total spleen RNA. Haemoglobin assays indicated that less than 15% of the spleen cells were lysed during the homogenization process, indicating that the majority of the spleen ′supernatant RNA′ was from the extracellular space of the organ rather than released by the splenocytes as a consequence of grinding. Quantitative RNA analysis showed that the ratio of spleen supernatant RNA/total RNA of BALB/c mice was inversely correlated with age ( from approximately 70% at 3 weeks to 45% at 6 months), but that of BXSB mice ( an animal model for systemic lupus erythematosus) remained at about 70% irrespective of age. Methyl Green-Pyronin Y staining of paraffin sections of mouse spleen revealed that extracellular RNA was distributed mainly in the sinuses of the organ. Culture supernatants of apoptotic splenocytes contained significant amounts of RNA, suggesting that the extracellular RNA in the spleen might have come from apoptotic lymphocytes. This is supported by the fact that ′thymus supernatant′ also contained significant amount of RNA. A possible correlation between spleen extracellular RNA and autoimmune diseases is discussed.

语种英语
WOS记录号WOS:000182581000003
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58125
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Zhao, WP,Liu, XG,Wang, W,et al. Large quantity of ribosomal RNA exists extracellularly in mouse spleen[J]. HISTOCHEMICAL JOURNAL,2002,34(8-9):391-396.
APA Zhao, WP,Liu, XG,Wang, W,&Gao, XM.(2002).Large quantity of ribosomal RNA exists extracellularly in mouse spleen.HISTOCHEMICAL JOURNAL,34(8-9),391-396.
MLA Zhao, WP,et al."Large quantity of ribosomal RNA exists extracellularly in mouse spleen".HISTOCHEMICAL JOURNAL 34.8-9(2002):391-396.
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