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学科主题基础医学
Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor
Han, HM; Wei, RS; Lai, AF; Yin, CC
关键词Ca2+ release channel ryanodine receptor hydrogen peroxide reactive oxygen redox-sensitive ion channel
刊名ACTA PHARMACOLOGICA SINICA
2006-07-01
DOI10.1111/j.1745-7254.2006.00386.x
27期:7页:888-894
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CALCIUM-RELEASE CHANNEL ; SKELETAL-MUSCLE ; SARCOPLASMIC-RETICULUM ; DIHYDROPYRIDINE RECEPTOR ; S-NITROSYLATION ; NITRIC-OXIDE ; ACTIVATION ; MODULATE ; TRIADIN ; OXYGEN
英文摘要

Aim: To elucidate the molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor (RyR1). Methods: Rabbit skeletal muscle sarcoplasmic reticulum was treated with hydrogen peroxide, then RyR1 complex was isolated. The proteins in the complex were analysed by electrophoresis, Western blot and electron microscopy. Results: (1) Hydrogen peroxide induces inter-subunit cross-linking within the tetrameric RyR1 molecule; (2) in parallel to inter-subunit cross-linking, the RyR1 molecule changes morphology; (3) the chemical and morphological changes are reversible: upon reduction by reducing agents, the RyR1 molecule regains its original state. Conclusion: These findings suggest that the molecular mechanism of RyR1 channel activity in sarcoplasmic reticulum regulated by hydrogen peroxide is through inter-subunit cross-linking within the tetrameric RyR1 molecule, which in turn induces structural changes of RyR1.

语种英语
WOS记录号WOS:000238485300016
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58137
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100083, Peoples R China
2.Univ Wales Coll Med, Heart Res Inst, Cardiff CF14 4XN, S Glam, Wales
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Han, HM,Wei, RS,Lai, AF,et al. Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor[J]. ACTA PHARMACOLOGICA SINICA,2006,27(7):888-894.
APA Han, HM,Wei, RS,Lai, AF,&Yin, CC.(2006).Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor.ACTA PHARMACOLOGICA SINICA,27(7),888-894.
MLA Han, HM,et al."Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor".ACTA PHARMACOLOGICA SINICA 27.7(2006):888-894.
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