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学科主题: 基础医学
题名:
Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor
作者: Han, HM; Wei, RS; Lai, AF; Yin, CC
关键词: Ca2+ release channel ; ryanodine receptor ; hydrogen peroxide ; reactive oxygen ; redox-sensitive ion channel
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2006-07-01
DOI: 10.1111/j.1745-7254.2006.00386.x
卷: 27, 期:7, 页:888-894
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CALCIUM-RELEASE CHANNEL ; SKELETAL-MUSCLE ; SARCOPLASMIC-RETICULUM ; DIHYDROPYRIDINE RECEPTOR ; S-NITROSYLATION ; NITRIC-OXIDE ; ACTIVATION ; MODULATE ; TRIADIN ; OXYGEN
英文摘要:

Aim: To elucidate the molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor (RyR1). Methods: Rabbit skeletal muscle sarcoplasmic reticulum was treated with hydrogen peroxide, then RyR1 complex was isolated. The proteins in the complex were analysed by electrophoresis, Western blot and electron microscopy. Results: (1) Hydrogen peroxide induces inter-subunit cross-linking within the tetrameric RyR1 molecule; (2) in parallel to inter-subunit cross-linking, the RyR1 molecule changes morphology; (3) the chemical and morphological changes are reversible: upon reduction by reducing agents, the RyR1 molecule regains its original state. Conclusion: These findings suggest that the molecular mechanism of RyR1 channel activity in sarcoplasmic reticulum regulated by hydrogen peroxide is through inter-subunit cross-linking within the tetrameric RyR1 molecule, which in turn induces structural changes of RyR1.

语种: 英语
WOS记录号: WOS:000238485300016
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58137
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100083, Peoples R China
2.Univ Wales Coll Med, Heart Res Inst, Cardiff CF14 4XN, S Glam, Wales

Recommended Citation:
Han, HM,Wei, RS,Lai, AF,et al. Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor[J]. ACTA PHARMACOLOGICA SINICA,2006,27(7):888-894.
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