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学科主题基础医学
Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells
Meng, Qingzhen; Jia, Zhuqing; Wang, Weiping; Li, Binhong; Ma, Kangtao; Zhou, Chunyan
关键词Id1 Wnt Signaling Cardiac Differentiation Proliferation P19cl6 Cells
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2011-08-26
DOI10.1016/j.bbrc.2011.07.079
412期:2页:253-259
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROSTATE-CANCER CELLS ; LOOP-HELIX PROTEINS ; TRANSCRIPTIONAL REGULATION ; GENE-EXPRESSION ; STEM-CELLS ; ID-1 ; APOPTOSIS ; PROMOTES ; ACTIVATION ; SURVIVAL
英文摘要

The inhibitor of DNA binding (Id) family of genes encodes negative regulators of basic helix-loop-helix transcription factors and has been implicated in such diverse cellular processes as differentiation, proliferation, apoptosis and migration. Id knockout mouse embryos display multiple cardiac defects but the specific role of Id1 in cardiac differentiation is unclear. In the present study, we investigated the function of Id1 in DMSO-induced P19CL6 cells, a widely-accepted cell model of cardiac differentiation. We found that Id1 was upregulated during the cardiac differentiation of P19CL6 cells. The expression of cardiac specific marker genes, Gata4, alpha-MHC and ISL1, was upregulated in P19CL6 cells stably transfected with 1d1 (P19CL6-Id1) during cardiac differentiation. The overexpression of Id1 reduced the number of cells in G1 phase and increased the cell population in G2, M and S phases, while knockdown of Id1 increased the number of cells in Cl phase from 48.6 +/- 2.51% to 62.2 +/- 1.52% at day 0 of cardiac induction, and from 52.5 +/- 3.41% to 63.7 +/- 1.02% at day 3 after cardiac induction, indicating that Id1 promoted proliferation of P19CL6 cells. Luciferase assays showed that the activity of TOP flash was higher in P19CL6-Id1 cells than wildtype P19CL6 cells, while Id1 expression was also upregulated in P19CL6 cells treated with Wnt3a or LiCI. This indicates that there may be positive feedback between Id1 and Wnt signaling which plays an important role in cardiac differentiation. (C) 2011 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000294594500011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58141
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位Peking Univ, Minist Educ China, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China
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GB/T 7714
Meng, Qingzhen,Jia, Zhuqing,Wang, Weiping,et al. Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,412(2):253-259.
APA Meng, Qingzhen,Jia, Zhuqing,Wang, Weiping,Li, Binhong,Ma, Kangtao,&Zhou, Chunyan.(2011).Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,412(2),253-259.
MLA Meng, Qingzhen,et al."Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 412.2(2011):253-259.
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