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学科主题临床医学
Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells
Yu, Jie1; Tong, Xunliang2; Li, Chengbin1; Huang, Yining2; Ye, Anpei1
刊名CHINESE OPTICS LETTERS
2013-09-01
DOI10.3788/COL201311.091701
11期:9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Optics
资助者National Basic Research Program of China ; National Science and Technology Infrastructure Program of China ; National Key Technologies R&amp ; D Program of China ; National Basic Research Program of China ; National Science and Technology Infrastructure Program of China ; National Key Technologies R&amp ; D Program of China
研究领域[WOS]Optics
关键词[WOS]DYNAMIC FORCE SPECTROSCOPY ; APOLIPOPROTEIN-A-I ; BINDING ; ADHESION ; CHOLESTEROL ; STRENGTH ; INTEGRIN ; REMOVAL ; MODELS
英文摘要

We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5+/-4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7+/-4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.

语种英语
所属项目编号2011CB809100 ; 2012BAF14B14 ; 2012ZX09303005003
资助者National Basic Research Program of China ; National Science and Technology Infrastructure Program of China ; National Key Technologies R&amp ; D Program of China ; National Basic Research Program of China ; National Science and Technology Infrastructure Program of China ; National Key Technologies R&amp ; D Program of China
WOS记录号WOS:000326416400020
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58163
专题北京大学第一临床医学院_神经内科
作者单位1.Peking Univ, Sch Elect Engn & Comp Sci, Key Lab Phys & Chem Nanodevices, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Neurol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Yu, Jie,Tong, Xunliang,Li, Chengbin,et al. Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells[J]. CHINESE OPTICS LETTERS,2013,11(9).
APA Yu, Jie,Tong, Xunliang,Li, Chengbin,Huang, Yining,&Ye, Anpei.(2013).Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells.CHINESE OPTICS LETTERS,11(9).
MLA Yu, Jie,et al."Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells".CHINESE OPTICS LETTERS 11.9(2013).
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