IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis
Wang, Lai1; Chen, Man1; Yuan, Lin1; Xiang, Yuting1; Zheng, Ruimao2; Zhu, Shigong1
关键词Mitochondrial Biogenesis 14 Mitochondrial Function 15-epoxyeicosatrienoic Acids Cortical Neuron Oxygen-glucose Deprivation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2014-07-18
DOI10.1016/j.bbrc.2014.06.022
450期:1页:604-609
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
资助者Major National Basic Research Grant of China ; National Natural Science Foundation of China ; Major National Basic Research Grant of China ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GLOBAL CEREBRAL-ISCHEMIA ; EPOXIDE HYDROLASE INHIBITOR ; AMYLOID-BETA ; PGC-1-ALPHA ; DYSFUNCTION ; STRESS ; RATS ; MICE ; REPERFUSION ; ACTIVATION
英文摘要

14,15-Epoxyeicosatrienoic acid (14,15-EET), a metabolite of arachidonic acid, is enriched in the brain cortex and exerts protective effect against neuronal apoptosis induced by ischemia/reperfusion. Although apoptosis has been well recognized to be closely associated with mitochondrial biogenesis and function, it is still unclear whether the neuroprotective effect of 14,15-EET is mediated by promotion of mitochondrial biogenesis and function in cortical neurons under the condition of oxygen-glucose deprivation (OGD). In this study, we found that 14,15-EET improved cell viability and inhibited apoptosis of cortical neurons. 14,15-EET significantly increased the mitochondrial mass and the ratio of mitochondrial DNA to nuclear DNA. Key makers of mitochondrial biogenesis, peroxisome proliferator activator receptor gamma-coactivator 1 alpha (PGC-1 alpha), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM), were elevated at both mRNA and protein levels in the cortical neurons treated with 14,15-EET. Moreover, 14,15-EET markedly attenuated the decline of mitochondrial membrane potential, reduced ROS, while increased ATP synthesis. Knockdown of cAMP-response element binding protein (CREB) by siRNA blunted the up-regulation of PGC-1 alpha cc and NRF-1 stimulated by 14,15-EET, and consequently abolished the neuroprotective effect of 14,15-EET. Our results indicate that 14,15-EET protects neurons from OGD-induced apoptosis by promoting mitochondrial biogenesis and function through CREB mediated activation of PGC-1 alpha and NRF-1. (C) 2014 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2010CB912504 ; 81171081
资助者Major National Basic Research Grant of China ; National Natural Science Foundation of China ; Major National Basic Research Grant of China ; National Natural Science Foundation of China
WOS记录号WOS:000343641000099
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58164
Collection北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Anat Histol & Embryol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lai,Chen, Man,Yuan, Lin,et al. 14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,450(1):604-609.
APA Wang, Lai,Chen, Man,Yuan, Lin,Xiang, Yuting,Zheng, Ruimao,&Zhu, Shigong.(2014).14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,450(1),604-609.
MLA Wang, Lai,et al."14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 450.1(2014):604-609.
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