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Studies on excretion kinetics of ten constituents in rat urine after oral administration of Shensong Yangxin Capsule by UPLC-MS/MS
Liu, Minyan1,2; Li, Song2; Zhao, Shaohua2; Wang, Hongtao2; Wang, Hairong2; Tu, Pengfei1
关键词UPLC-MS MS urinary excretion kinetics multiple-reaction monitoring solid-phase extraction Shensong Yangxin Capsule
刊名BIOMEDICAL CHROMATOGRAPHY
2014-04-01
DOI10.1002/bmc.3064
28期:4页:525-533
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
资助者National Key Basic Research Program of China (973 Program) ; National Great Scientific and Technological Project of China ; National Key Basic Research Program of China (973 Program) ; National Great Scientific and Technological Project of China
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TANDEM MASS-SPECTROMETRY ; RADIX-PAEONIAE RUBRA ; LIQUID-CHROMATOGRAPHY ; QUANTITATIVE-DETERMINATION ; PLASMA ; IDENTIFICATION ; JATRORRHIZINE ; PAEONIFLORIN ; ALBIFLORIN ; BERBERINE
英文摘要

A rapid and sensitive ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of 10 major active constituents in rat urine after oral administration of Shensong Yangxin Capsule (SSYX) using diazepam as an internal standard (IS). The urine samples were pretreated and extracted by solid-phase extraction prior to UPLC. Chromatographic separation was achieved on a Waters C-18 (2.1x50mm, 1.7 mu m) column using a gradient elution program with 0.1% formic acid aqueous solution and acetonitrile at a flow rate of 0.4mL/min. Detection and quantitation were accomplished by a hybrid quadrupole mass spectrometer using electrospray ionization source and multiple reaction monitoring in the positive ionization mode. The mass transition ion-pairs (m/z) for quantitation were all optimized and the total run time was 4.50min. The specificity, linearity, accuracy, precision, recovery, matrix effect and stabilities were all validated for the analytes in urine samples. The validation results indicated that this method was simple, rapid, specific and reliable. The proposed method was successfully applied to investigate the urinary excretion kinetics of 10 compounds in rat after oral administration of SSYX. Copyright (c) 2013 John Wiley & Sons, Ltd.

语种英语
所属项目编号2012CB518606 ; 2011ZX09201-201-28
资助者National Key Basic Research Program of China (973 Program) ; National Great Scientific and Technological Project of China ; National Key Basic Research Program of China (973 Program) ; National Great Scientific and Technological Project of China
WOS记录号WOS:000334331700011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58199
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shijiazhuang Yiling Pharmaceut Co Ltd, Shijiazhuang 050035, Peoples R China
推荐引用方式
GB/T 7714
Liu, Minyan,Li, Song,Zhao, Shaohua,et al. Studies on excretion kinetics of ten constituents in rat urine after oral administration of Shensong Yangxin Capsule by UPLC-MS/MS[J]. BIOMEDICAL CHROMATOGRAPHY,2014,28(4):525-533.
APA Liu, Minyan,Li, Song,Zhao, Shaohua,Wang, Hongtao,Wang, Hairong,&Tu, Pengfei.(2014).Studies on excretion kinetics of ten constituents in rat urine after oral administration of Shensong Yangxin Capsule by UPLC-MS/MS.BIOMEDICAL CHROMATOGRAPHY,28(4),525-533.
MLA Liu, Minyan,et al."Studies on excretion kinetics of ten constituents in rat urine after oral administration of Shensong Yangxin Capsule by UPLC-MS/MS".BIOMEDICAL CHROMATOGRAPHY 28.4(2014):525-533.
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