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学科主题: 临床医学
题名:
Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro
作者: Zhu, Ming-Xia1,2,4; Wan, Wen-Li1,2,4; Li, Hai-Shen1,2; Wang, Jing1,2; Chen, Gui-An3,4; Ke, Xiao-Yan1,2,4
关键词: Human embryonic stem cells\ ; Hematopoietic progenitor cells ; T lymphocytes ; Cell differentiation ; Thymopentin
刊名: EXPERIMENTAL CELL RESEARCH
发表日期: 2015-02-15
DOI: 10.1016/j.yexcr.2014.12.012
卷: 331, 期:2, 页:387-398
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: HEMATOPOIETIC PROGENITORS ; DIFFERENTIATION CULTURES ; LYMPHOID PROGENITORS ; HUMAN THYMUS ; EXPRESSION ; MODEL ; ESTABLISHMENT ; MACROPHAGES ; MATURATION ; INDUCTION
英文摘要:

Thymopentin is a group of biologically active peptide secreted mainly by the epithelial cells of thymic cortex and medulla. Whether it promotes T cells production from human embryonic stem cells(hESCs) in vitro remains an elusive issue. In the present study, we develop a novel strategy that enhances T-cell lineage differentiation of hESCs in collagen matrix culture by sequential cytokine cocktails treatment combined with thymopentin stimulation. We observed that approximately 30.75% cells expressed CD34 on day 14 of the cultures and expressed the surface markers of erythroid, lymphoid and myeloid lineages. The results of colony assays and gene expressions by RT-PCR analysis also demonstrated that hematopoietic progenitor cells (HPCs) derived from hESCs were capable of multi-lineage differentiation. Further study revealed that culturing with thymopentin treatment, the CD34(+)CD45RA(+)CD7(+) cells sorted from HPCs expressed T-cell-related genes, IKAROS, DNTT, TCR gamma and TCR beta and T-cell surface markers, CD3, cytoplasmic CD3, CD5, CD27, TCR gamma delta, CD4 and CD8. The differentiated cells produced the cytokines including IFN-gamma, IL-2 and TNF-alpha in response to stimulation, providing the evidence for T-cell function of these cells. In conclusion, thymopentin enhances T-cell lineage differentiation from hESCs in vitro by mimicking thymus peptide environment in vivo. (C) 2015 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81072256 ; 20120001120132
项目资助者: Natural Science Foundation of China ; Doctoral Fund for New Scholars of the Education of Ministry of China
WOS记录号: WOS:000349594200013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58201
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Lymphoma Res Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Reprod Med Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 3, Dept Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhu, Ming-Xia,Wan, Wen-Li,Li, Hai-Shen,et al. Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro[J]. EXPERIMENTAL CELL RESEARCH,2015,331(2):387-398.
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