北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 血液内科  > 期刊论文
学科主题: 临床医学
题名:
Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro
作者: Zhu, Ming-Xia1,2,4; Wan, Wen-Li1,2,4; Li, Hai-Shen1,2; Wang, Jing1,2; Chen, Gui-An3,4; Ke, Xiao-Yan1,2,4
关键词: Human embryonic stem cells\ ; Hematopoietic progenitor cells ; T lymphocytes ; Cell differentiation ; Thymopentin
刊名: EXPERIMENTAL CELL RESEARCH
发表日期: 2015-02-15
DOI: 10.1016/j.yexcr.2014.12.012
卷: 331, 期:2, 页:387-398
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: HEMATOPOIETIC PROGENITORS ; DIFFERENTIATION CULTURES ; LYMPHOID PROGENITORS ; HUMAN THYMUS ; EXPRESSION ; MODEL ; ESTABLISHMENT ; MACROPHAGES ; MATURATION ; INDUCTION
英文摘要:

Thymopentin is a group of biologically active peptide secreted mainly by the epithelial cells of thymic cortex and medulla. Whether it promotes T cells production from human embryonic stem cells(hESCs) in vitro remains an elusive issue. In the present study, we develop a novel strategy that enhances T-cell lineage differentiation of hESCs in collagen matrix culture by sequential cytokine cocktails treatment combined with thymopentin stimulation. We observed that approximately 30.75% cells expressed CD34 on day 14 of the cultures and expressed the surface markers of erythroid, lymphoid and myeloid lineages. The results of colony assays and gene expressions by RT-PCR analysis also demonstrated that hematopoietic progenitor cells (HPCs) derived from hESCs were capable of multi-lineage differentiation. Further study revealed that culturing with thymopentin treatment, the CD34(+)CD45RA(+)CD7(+) cells sorted from HPCs expressed T-cell-related genes, IKAROS, DNTT, TCR gamma and TCR beta and T-cell surface markers, CD3, cytoplasmic CD3, CD5, CD27, TCR gamma delta, CD4 and CD8. The differentiated cells produced the cytokines including IFN-gamma, IL-2 and TNF-alpha in response to stimulation, providing the evidence for T-cell function of these cells. In conclusion, thymopentin enhances T-cell lineage differentiation from hESCs in vitro by mimicking thymus peptide environment in vivo. (C) 2015 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81072256 ; 20120001120132
项目资助者: Natural Science Foundation of China ; Doctoral Fund for New Scholars of the Education of Ministry of China
WOS记录号: WOS:000349594200013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58201
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Lymphoma Res Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Reprod Med Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 3, Dept Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhu, Ming-Xia,Wan, Wen-Li,Li, Hai-Shen,et al. Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro[J]. EXPERIMENTAL CELL RESEARCH,2015,331(2):387-398.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhu, Ming-Xia]'s Articles
[Wan, Wen-Li]'s Articles
[Li, Hai-Shen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhu, Ming-Xia]‘s Articles
[Wan, Wen-Li]‘s Articles
[Li, Hai-Shen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace