北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科  > 期刊论文
学科主题: 临床医学
题名:
Anti-inflammatory macrophages activate invasion in pancreatic adenocarcinoma by increasing the MMP9 and ADAM8 expression
作者: Puolakkainen, Pauli1; Koski, Aino1; Vainionpaa, Sanna1; Shen, Zhanlong2,3; Repo, Heikki4; Kemppainen, Esko1; Mustonen, Harri1; Seppanen, Hanna1
关键词: Pancreatic cancer ; Tumor-associated macrophages ; Hypoxia ; ADAM8 expression
刊名: MEDICAL ONCOLOGY
发表日期: 2014-03-01
DOI: 10.1007/s12032-014-0884-9
卷: 31, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TUMOR-ASSOCIATED MACROPHAGES ; NF-KAPPA-B ; GASTRIC-CANCER ; DUCTAL ADENOCARCINOMA ; CELLS ; GROWTH ; MATRIX ; CARCINOMA ; PATHWAYS ; MATRIX-METALLOPROTEINASE-9
英文摘要:

Patients with chronic pancreatitis with local inflammation have high risk for pancreatic cancer. The aim of this study was to examine the role of the inflammatory cells in the invasion of pancreatic cancer cells, focusing on the involvement of a disintegrin and metalloproteinase 8 (ADAM8) and matrix metalloproteinase 9 (MMP9) proteins. ADAM8 expression is associated with worse survival of pancreatic cancer patients. Monocytes from healthy donors were differentiated into macrophages. Pancreatic adenocarcinoma cells were cultured either alone or with differentiated macrophages. The cancer cell migration rate in Matrigel was measured by imaging fluorescently stained cells for 24 h. After invasion, cells were sorted into CD14 positive/negative macrophages and cancer cells with magnetic separation. The expression of ADAM8 and MMP9 was measured by the real-time PCR. Protein-level expression of ADAM8 and MMP9 was analyzed by Western blotting. In two series, siRNA technique was used to reduce either ADAM8 or MMP9 expression in the cancer cells. The coculture with macrophages increased cancer cell migration rate in Matrigel, and increased ADAM8 and MMP9 mRNA expression and protein level in the cancer cells. Reduction of ADAM8 expression with siRNA in the cancer cells decreased macrophage-induced migration rate of the cancer cells from 11.7 +/- 0.3 mu m/h to 9.0 +/- 0.2 mu m/h (p < 0.01), and reduction of MMP9 expression decreased the migration rate to 10.1 +/- 0.2 mu m/h (p < 0.01). Antiinflammatory macrophages increase pancreatic cancer cell migration rate in basement membrane matrix by inducing ADAM8 and MMP9 expression in cancer cells, thereby possibly enhancing the invasiveness of cancer.

语种: 英语
项目资助者: Georg C Ehrnrooth foundation ; Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds
WOS记录号: WOS:000337728700042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58210
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Helsinki, Dept Surg, Helsinki Univ Cent Hosp, Helsinki 00029, Finland
2.Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing, Peoples R China
3.Peking Univ Peoples Hosp, Dept Surg Oncol, Beijing, Peoples R China
4.Univ Helsinki, Dept Bacteriol & Immunol, Hartmann Inst, Helsinki 00029, Finland

Recommended Citation:
Puolakkainen, Pauli,Koski, Aino,Vainionpaa, Sanna,et al. Anti-inflammatory macrophages activate invasion in pancreatic adenocarcinoma by increasing the MMP9 and ADAM8 expression[J]. MEDICAL ONCOLOGY,2014,31(3).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Puolakkainen, Pauli]'s Articles
[Koski, Aino]'s Articles
[Vainionpaa, Sanna]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Puolakkainen, Pauli]‘s Articles
[Koski, Aino]‘s Articles
[Vainionpaa, Sanna]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace