学科主题基础医学
Characterization of a novel positive transcription regulatory element that differentially regulates the alpha-2-macroglobulin gene in replicative senescence
Li, Renzhong; Ma, Liwei; Huang, Yu; Zhang, Zongyu; Tong, Tanjun
关键词Alpha-2-macroglobulin Senescence ATEE
刊名BIOGERONTOLOGY
2011-12-01
DOI10.1007/s10522-011-9339-2
12期:6页:517-525
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Geriatrics & Gerontology
研究领域[WOS]Geriatrics & Gerontology
关键词[WOS]BETA-AMYLOID PEPTIDE ; BINDING-PROTEIN ; GROWTH-FACTOR ; OLD RATS ; ALPHA(2)-MACROGLOBULIN ; EXPRESSION ; FIBROBLASTS ; WILLIS ; CIRCLE ; STAT3
英文摘要

Alpha-2-macroglobulin (alpha 2M), a protease inhibitor, is implicated in Alzheimer′s disease, atherosclerosis, and other age-related diseases. The elevated level of alpha 2M mRNA has been described in replicative senescence and it could be used as a biomarker of the aging cells. However, the mechanism responsible for the up-regulation of its expression is still unclear. This report identified a novel transcriptional regulatory element, the alpha 2M transcription enhancement element (ATEE), within the alpha 2M promoter. This element differentially activates alpha 2M expression in senescent versus young fibroblasts. Electrophoretic mobility shift assays revealed abundant complexes in senescent cell nuclear extracts compared with young cell nuclear extracts. The DNase I footprint revealed the protein-binding core sequence through which the protein binds the ATEE. Mutation within ATEE selectively abolished alpha 2M promoter activity in senescent (but not young) cells. These results indicated the ATEE, as a positive transcription regulatory element, contributes to the up-regulation of alpha 2M during replicative senescence.

语种英语
WOS记录号WOS:000296633300003
项目编号2007CB507400 ; 30973146 ; 31000609 ; 20090001120044
资助机构National Basic Research Programs of China ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58225
专题北京大学基础医学院_北京大学衰老研究中心
北京大学基础医学院
作者单位Peking Univ, Hlth Sci Ctr, Res Ctr Aging, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Renzhong,Ma, Liwei,Huang, Yu,et al. Characterization of a novel positive transcription regulatory element that differentially regulates the alpha-2-macroglobulin gene in replicative senescence[J]. BIOGERONTOLOGY,2011,12(6):517-525.
APA Li, Renzhong,Ma, Liwei,Huang, Yu,Zhang, Zongyu,&Tong, Tanjun.(2011).Characterization of a novel positive transcription regulatory element that differentially regulates the alpha-2-macroglobulin gene in replicative senescence.BIOGERONTOLOGY,12(6),517-525.
MLA Li, Renzhong,et al."Characterization of a novel positive transcription regulatory element that differentially regulates the alpha-2-macroglobulin gene in replicative senescence".BIOGERONTOLOGY 12.6(2011):517-525.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
art%3A10.1007%2Fs105(360KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Renzhong]的文章
[Ma, Liwei]的文章
[Huang, Yu]的文章
百度学术
百度学术中相似的文章
[Li, Renzhong]的文章
[Ma, Liwei]的文章
[Huang, Yu]的文章
必应学术
必应学术中相似的文章
[Li, Renzhong]的文章
[Ma, Liwei]的文章
[Huang, Yu]的文章
相关权益政策
暂无数据
收藏/分享
文件名: art%3A10.1007%2Fs10522-011-9339-2.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。