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学科主题: 基础医学
题名:
Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
作者: Dong, Rong1,2; Long, Jinhua3; Xu, Xiaoli1,2; Zhang, Chunlin2; Wen, Zongyao5; Li, Long4; Yao, Weijuan5; Zeng, Zhu1,2,6
关键词: Dendritic cell ; Fourier transformed infrared spectroscopy ; Functional states of cells ; NF-kappa B
刊名: BIOMEDICAL ENGINEERING ONLINE
发表日期: 2014-01-10
DOI: 10.1186/1475-925X-13-2
卷: 13
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical
研究领域[WOS]: Engineering
关键词[WOS]: NF-KAPPA-B ; RNA INTERFERENCE ; TUMOR-CELLS ; CANCER ; RELB ; IMMUNOTHERAPY ; TOLERANCE ; IMMUNITY ; VIRUS ; FIBROBLASTS
英文摘要:

Background: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states.

Methods: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods.

Results: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-kappa B) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-kappa B (R-2:0.69 and R-2:0.81, respectively).

Conclusion: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-kappa B, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It′s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.

语种: 英语
所属项目编号: 11162003 ; 31260227 ; 210196 ; 2011-24 ; GZ-SY- [2011]3065 ; 2009-79 ; J-2008-2274 ; 2013-8
项目资助者: National Natural Science Foundations of China ; Key Project of Chinese Ministry of Education ; Supporting Project of Distinguished Young Science and Technology Scholars of Guizhou Province ; Offends Pass Item of Science and Technology for Social Development in Guizhou Province ; Special Foundation of Governor of Guizhou Province ; Foundation of Science and Technology of Guizhou Province ; Technology Foundation for Selected Overseas Chinese of Guizhou Province
WOS记录号: WOS:000329912500001
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58236
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Guiyang Med Coll, Dept Biomed Engn, Guiyang 550004, Guizhou, Peoples R China
2.Guiyang Med Coll, Dept Cell Biol, Guiyang 550004, Guizhou, Peoples R China
3.Guiyang Med Coll, Affiliated Canc Hosp, Dept Head & Neck, Guiyang 550004, Guizhou, Peoples R China
4.Guiyang Med Coll, Affiliated Hosp, Dept Nephrol, Guiyang 550004, Guizhou, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Hemorheol, Beijing 100083, Peoples R China
6.Guiyang Med Coll, Dept Biotechnol, Guiyang 550004, Guizhou, Peoples R China

Recommended Citation:
Dong, Rong,Long, Jinhua,Xu, Xiaoli,et al. Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy[J]. BIOMEDICAL ENGINEERING ONLINE,2014,13.
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