IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
Dong, Rong1,2; Long, Jinhua3; Xu, Xiaoli1,2; Zhang, Chunlin2; Wen, Zongyao5; Li, Long4; Yao, Weijuan5; Zeng, Zhu1,2,6
关键词Dendritic cell Fourier transformed infrared spectroscopy Functional states of cells NF-kappa B
刊名BIOMEDICAL ENGINEERING ONLINE
2014-01-10
DOI10.1186/1475-925X-13-2
13
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical
研究领域[WOS]Engineering
关键词[WOS]NF-KAPPA-B ; RNA INTERFERENCE ; TUMOR-CELLS ; CANCER ; RELB ; IMMUNOTHERAPY ; TOLERANCE ; IMMUNITY ; VIRUS ; FIBROBLASTS
英文摘要

Background: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states.

Methods: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods.

Results: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-kappa B) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-kappa B (R-2:0.69 and R-2:0.81, respectively).

Conclusion: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-kappa B, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It′s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.

语种英语
WOS记录号WOS:000329912500001
项目编号11162003 ; 31260227 ; 210196 ; 2011-24 ; GZ-SY- [2011]3065 ; 2009-79 ; J-2008-2274 ; 2013-8
资助机构National Natural Science Foundations of China ; Key Project of Chinese Ministry of Education ; Supporting Project of Distinguished Young Science and Technology Scholars of Guizhou Province ; Offends Pass Item of Science and Technology for Social Development in Guizhou Province ; Special Foundation of Governor of Guizhou Province ; Foundation of Science and Technology of Guizhou Province ; Technology Foundation for Selected Overseas Chinese of Guizhou Province
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58236
专题北京大学基础医学院
作者单位1.Guiyang Med Coll, Dept Biomed Engn, Guiyang 550004, Guizhou, Peoples R China
2.Guiyang Med Coll, Dept Cell Biol, Guiyang 550004, Guizhou, Peoples R China
3.Guiyang Med Coll, Affiliated Canc Hosp, Dept Head & Neck, Guiyang 550004, Guizhou, Peoples R China
4.Guiyang Med Coll, Affiliated Hosp, Dept Nephrol, Guiyang 550004, Guizhou, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Hemorheol, Beijing 100083, Peoples R China
6.Guiyang Med Coll, Dept Biotechnol, Guiyang 550004, Guizhou, Peoples R China
推荐引用方式
GB/T 7714
Dong, Rong,Long, Jinhua,Xu, Xiaoli,et al. Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy[J]. BIOMEDICAL ENGINEERING ONLINE,2014,13.
APA Dong, Rong.,Long, Jinhua.,Xu, Xiaoli.,Zhang, Chunlin.,Wen, Zongyao.,...&Zeng, Zhu.(2014).Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy.BIOMEDICAL ENGINEERING ONLINE,13.
MLA Dong, Rong,et al."Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy".BIOMEDICAL ENGINEERING ONLINE 13(2014).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Investigations of th(98KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dong, Rong]的文章
[Long, Jinhua]的文章
[Xu, Xiaoli]的文章
百度学术
百度学术中相似的文章
[Dong, Rong]的文章
[Long, Jinhua]的文章
[Xu, Xiaoli]的文章
必应学术
必应学术中相似的文章
[Dong, Rong]的文章
[Long, Jinhua]的文章
[Xu, Xiaoli]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。