Institutional Repository of Peking University School of Basic Medical Sciences
|Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy|
|Dong, Rong1,2; Long, Jinhua3; Xu, Xiaoli1,2; Zhang, Chunlin2; Wen, Zongyao5; Li, Long4; Yao, Weijuan5; Zeng, Zhu1,2,6|
|关键词||Dendritic cell Fourier transformed infrared spectroscopy Functional states of cells NF-kappa B|
|刊名||BIOMEDICAL ENGINEERING ONLINE|
|WOS标题词||Science & Technology|
|关键词[WOS]||NF-KAPPA-B ; RNA INTERFERENCE ; TUMOR-CELLS ; CANCER ; RELB ; IMMUNOTHERAPY ; TOLERANCE ; IMMUNITY ; VIRUS ; FIBROBLASTS|
Background: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states.
Methods: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods.
Results: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-kappa B) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-kappa B (R-2:0.69 and R-2:0.81, respectively).
Conclusion: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-kappa B, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It′s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.
|项目编号||11162003 ; 31260227 ; 210196 ; 2011-24 ; GZ-SY- 3065 ; 2009-79 ; J-2008-2274 ; 2013-8|
|资助机构||National Natural Science Foundations of China ; Key Project of Chinese Ministry of Education ; Supporting Project of Distinguished Young Science and Technology Scholars of Guizhou Province ; Offends Pass Item of Science and Technology for Social Development in Guizhou Province ; Special Foundation of Governor of Guizhou Province ; Foundation of Science and Technology of Guizhou Province ; Technology Foundation for Selected Overseas Chinese of Guizhou Province|
|作者单位||1.Guiyang Med Coll, Dept Biomed Engn, Guiyang 550004, Guizhou, Peoples R China|
2.Guiyang Med Coll, Dept Cell Biol, Guiyang 550004, Guizhou, Peoples R China
3.Guiyang Med Coll, Affiliated Canc Hosp, Dept Head & Neck, Guiyang 550004, Guizhou, Peoples R China
4.Guiyang Med Coll, Affiliated Hosp, Dept Nephrol, Guiyang 550004, Guizhou, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Hemorheol, Beijing 100083, Peoples R China
6.Guiyang Med Coll, Dept Biotechnol, Guiyang 550004, Guizhou, Peoples R China
|Dong, Rong,Long, Jinhua,Xu, Xiaoli,et al. Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy[J]. BIOMEDICAL ENGINEERING ONLINE,2014,13.|
|APA||Dong, Rong.,Long, Jinhua.,Xu, Xiaoli.,Zhang, Chunlin.,Wen, Zongyao.,...&Zeng, Zhu.(2014).Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy.BIOMEDICAL ENGINEERING ONLINE,13.|
|MLA||Dong, Rong,et al."Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy".BIOMEDICAL ENGINEERING ONLINE 13(2014).|
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