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学科主题基础医学
Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)
Lau, Lok-Ting1,2,3; Reid, Scott M.4; King, Donald P.4; Lau, Anson Ming-Fung1; Shaw, Andrew E.4; Ferris, Nigel P.4; Yu, Albert Cheung-Hoi1,2,3
关键词Foot-and-mouth Disease Virus Diagnosis Nasba-electrochemiluminescence Nasba-enzyme-linked Oligonucleotide Capture Real-time Rt-pcr
刊名VETERINARY MICROBIOLOGY
2008
DOI10.1016/j.vetmic.2007.07.008
126期:1-3页:101-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology ; Veterinary Sciences
研究领域[WOS]Microbiology ; Veterinary Sciences
关键词[WOS]LABORATORY DIAGNOSIS ; ASSAY
英文摘要

A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1 % of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV. (C) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000251924700011
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58237
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Inst Anim Hlth, Woking GU24 0NF, Surrey, England
4.8 F Hang Tung Resources Ctr, Hai Kang Life Corp Ltd, Hong Kong, Hong Kong, Peoples R China
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GB/T 7714
Lau, Lok-Ting,Reid, Scott M.,King, Donald P.,et al. Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)[J]. VETERINARY MICROBIOLOGY,2008,126(1-3):101-110.
APA Lau, Lok-Ting.,Reid, Scott M..,King, Donald P..,Lau, Anson Ming-Fung.,Shaw, Andrew E..,...&Yu, Albert Cheung-Hoi.(2008).Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA).VETERINARY MICROBIOLOGY,126(1-3),101-110.
MLA Lau, Lok-Ting,et al."Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)".VETERINARY MICROBIOLOGY 126.1-3(2008):101-110.
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