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学科主题: 基础医学
题名:
Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)
作者: Lau, Lok-Ting1,2,3; Reid, Scott M.4; King, Donald P.4; Lau, Anson Ming-Fung1; Shaw, Andrew E.4; Ferris, Nigel P.4; Yu, Albert Cheung-Hoi1,2,3
关键词: foot-and-mouth disease virus ; diagnosis ; NASBA-electrochemiluminescence ; NASBA-enzyme-linked oligonucleotide capture ; real-time RT-PCR
刊名: VETERINARY MICROBIOLOGY
发表日期: 2008
DOI: 10.1016/j.vetmic.2007.07.008
卷: 126, 期:1-3, 页:101-110
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology ; Veterinary Sciences
研究领域[WOS]: Microbiology ; Veterinary Sciences
关键词[WOS]: LABORATORY DIAGNOSIS ; ASSAY
英文摘要:

A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1 % of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV. (C) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000251924700011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58237
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Inst Anim Hlth, Woking GU24 0NF, Surrey, England
4.8 F Hang Tung Resources Ctr, Hai Kang Life Corp Ltd, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Lau, Lok-Ting,Reid, Scott M.,King, Donald P.,et al. Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)[J]. VETERINARY MICROBIOLOGY,2008,126(1-3):101-110.
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