北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells
作者: Gao, BB; Lei, BL; Zhang, YY; Han, QD
关键词: alpha-1 adrenergic receptors ; norepinephrine ; cell division ; Ca2+-calmodulin dependent protein kinase ; HEK293 cells ; radioligand assay ; plasmids ; recombinant DNA ; propranolol ; thymidine
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2000
卷: 21, 期:1, 页:55-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SMOOTH-MUSCLE CELLS ; SUBTYPES ; GROWTH
英文摘要:

AIM: To examine the ability of (alpha(1)-AR subtypes on proliferation and Ca2+ -calmodulin dependent protein kinase(CCDPK, formerly called MAPK) activation in transfected human embryo kidney 293 (HEK293) cells. METHODS: pREP8/alpha(1A)-AR, pREP4/alpha(1B)-AR, and pREP9/(alpha 1D)-AR were transfected, respectively, into HEK293 cells by calcium phosphate precipitation. The expression of alpha(1)-AR was detected by radioligand binding assays. DNA synthesis was measured by [H-3]thymidine incorporation. CCDPK activity was determined by immunoprecipitation method and myelin basic protein was used as substrate. RESULTS: Three clonal HEK293 cell lines stably expressing alpha(1A)-or alpha(1B)- or alpha(1D)-AR were chosen and characterized by radioligand binding assay with receptor densities of about 0.6 nmol.g(-1). Treatment with norepinephrine (NE) in the presence of propranolol for 24 h increased DNA synthesis in HEK293/alpha(1A)- or HEK293/alpha(1B)-AR cells concentration-dependently, with EC50 values of 48.8 nmol.L-1(95 % confidence limits 9.7 - 246 nmol L-1) and 8.4 nmol.L-1 (95 % confidence Limits 2.1-32.9 nmol L-1), respectively. The increase of DNA synthesis induced by NE 10 mu mol.L-1 was 201 % +/- 28 % and 269 % +/- 44! % of basal, and the activation of CCDPK was 171 % +/- 84 % and 292 % +/- 92 % of basal in HEK293/alpha(1A)-AR and HEK293/alpha(1B)-AR cells, respectively. Preincubation with prazosin completely abolished NE-induced CCDPK activation in HEK293/alpha(1A)- and alpha(1B)-AR cells. Those changes were not found in HEK293/alpha(1D)-AR cells. CONCLUSION: The activation of alpha(1A)- or alpha(1B)-AR but not alpha(1D)-AK induces cell proliferation.

语种: 英语
WOS记录号: WOS:000084710700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58242
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Beijing Med Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China

Recommended Citation:
Gao, BB,Lei, BL,Zhang, YY,et al. Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(1):55-59.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, BB]'s Articles
[Lei, BL]'s Articles
[Zhang, YY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, BB]‘s Articles
[Lei, BL]‘s Articles
[Zhang, YY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace