IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells
Gao, BB; Lei, BL; Zhang, YY; Han, QD
关键词alpha-1 adrenergic receptors norepinephrine cell division Ca2+-calmodulin dependent protein kinase HEK293 cells radioligand assay plasmids recombinant DNA propranolol thymidine
刊名ACTA PHARMACOLOGICA SINICA
2000
21期:1页:55-59
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SMOOTH-MUSCLE CELLS ; SUBTYPES ; GROWTH
英文摘要

AIM: To examine the ability of (alpha(1)-AR subtypes on proliferation and Ca2+ -calmodulin dependent protein kinase(CCDPK, formerly called MAPK) activation in transfected human embryo kidney 293 (HEK293) cells. METHODS: pREP8/alpha(1A)-AR, pREP4/alpha(1B)-AR, and pREP9/(alpha 1D)-AR were transfected, respectively, into HEK293 cells by calcium phosphate precipitation. The expression of alpha(1)-AR was detected by radioligand binding assays. DNA synthesis was measured by [H-3]thymidine incorporation. CCDPK activity was determined by immunoprecipitation method and myelin basic protein was used as substrate. RESULTS: Three clonal HEK293 cell lines stably expressing alpha(1A)-or alpha(1B)- or alpha(1D)-AR were chosen and characterized by radioligand binding assay with receptor densities of about 0.6 nmol.g(-1). Treatment with norepinephrine (NE) in the presence of propranolol for 24 h increased DNA synthesis in HEK293/alpha(1A)- or HEK293/alpha(1B)-AR cells concentration-dependently, with EC50 values of 48.8 nmol.L-1(95 % confidence limits 9.7 - 246 nmol L-1) and 8.4 nmol.L-1 (95 % confidence Limits 2.1-32.9 nmol L-1), respectively. The increase of DNA synthesis induced by NE 10 mu mol.L-1 was 201 % +/- 28 % and 269 % +/- 44! % of basal, and the activation of CCDPK was 171 % +/- 84 % and 292 % +/- 92 % of basal in HEK293/alpha(1A)-AR and HEK293/alpha(1B)-AR cells, respectively. Preincubation with prazosin completely abolished NE-induced CCDPK activation in HEK293/alpha(1A)- and alpha(1B)-AR cells. Those changes were not found in HEK293/alpha(1D)-AR cells. CONCLUSION: The activation of alpha(1A)- or alpha(1B)-AR but not alpha(1D)-AK induces cell proliferation.

语种英语
WOS记录号WOS:000084710700008
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58242
Collection北京大学第三临床医学院_心血管内科
作者单位Beijing Med Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Gao, BB,Lei, BL,Zhang, YY,et al. Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(1):55-59.
APA Gao, BB,Lei, BL,Zhang, YY,&Han, QD.(2000).Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells.ACTA PHARMACOLOGICA SINICA,21(1),55-59.
MLA Gao, BB,et al."Cell proliferation and Ca2+-calmodulin dependent protein kinase activation mediated by alpha(1A)- and alpha(1B)-adrenergic receptor in HEK293 cells".ACTA PHARMACOLOGICA SINICA 21.1(2000):55-59.
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