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学科主题临床医学
Epigenetic and genetic alterations of the EDNRB gene in nasopharyngeal carcinoma
Zhou, Liang1,3,4; Feng, Xiangling1; Shan, Wenjiao1; Zhou, Wen1; Liu, Weidong1; Wang, Lei1; Zhu, Bin1; Yi, Hongmei1,2; Yao, Kaitai1,5; Ren, Caiping1
关键词EDNRB expression hypermethylation loss of heterozygosity nasopharyngeal carcinoma
刊名ONCOLOGY
2007
DOI10.1159/000113146
72期:5-6页:357-363
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]ENDOTHELIN-B RECEPTOR ; COMPARATIVE GENOMIC HYBRIDIZATION ; CANDIDATE TUMOR-SUPPRESSOR ; STRESS-RESPONSIVE GENE ; PROMOTER HYPERMETHYLATION ; BLADDER-CANCER ; METHYLATION ; 3P21.3 ; AGE ; INACTIVATION
英文摘要

Background: Loss of heterozygosity (LOH) at 13q22 is a common event in nasopharyngeal carcinoma (NPC). EDNRB gene located at 13q22 has been demonstrated to be hypermethylated in some kinds of tumors. In the current study, we focused on the epigenetic and genetic alterations of EDNRB in NPC. Methods: The mRNA expression of EDNRB was detected by semiquantitative RT-PCR and real-time quantitative PCR in 49 NPC and 12 chronic nasopharyngitis biopsies. The methylation and LOH status of EDNRB were examined by methylation-specific polymerase chain reaction, microsatellite PCR and sequencing. We also examined the mRNA expression of EDNRB in four NPC cell lines after 5-aza-2′-deoxycytidine treatment. Results: EDNRB was downregulated in primary NPC tissues and NPC cell lines, and a relatively higher methylation level of EDNRB was found in NPC biopsies (84%) compared to that in chronic nasopharyngitis biopsies (42%). Treatment of NPC cell lines with 5-aza-2′-deoxycytidine activated EDNRB expression. LOH of EDNRB gene was also found at two microsatellite sites with ratios of 6.25 and 16.67% in NPC. Conclusion: Our results suggested that EDNRB expression may be affected by aberrant promoter methylation and gene deletion and may play a role in the development of NPC. Copyright (C) 2008 S. Karger AG, Basel.

语种英语
WOS记录号WOS:000252590200016
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58245
专题北京大学深圳医院
作者单位1.Cent S Univ, Xiang Ya Sch Med, Inst Canc Res, Changsha 4100780, Hunan, Peoples R China
2.Hunan Province Peoples Hosp, Inst Clin Med, Changsha, Peoples R China
3.Peking Univ, Med Ctr, Shenzen Hosp, Shenzhen, Peoples R China
4.Hong Kong Univ Sci & Technol, Shenzhen, Peoples R China
5.So Med Univ, Inst Canc Res, Guangzhou, Peoples R China
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GB/T 7714
Zhou, Liang,Feng, Xiangling,Shan, Wenjiao,et al. Epigenetic and genetic alterations of the EDNRB gene in nasopharyngeal carcinoma[J]. ONCOLOGY,2007,72(5-6):357-363.
APA Zhou, Liang.,Feng, Xiangling.,Shan, Wenjiao.,Zhou, Wen.,Liu, Weidong.,...&Ren, Caiping.(2007).Epigenetic and genetic alterations of the EDNRB gene in nasopharyngeal carcinoma.ONCOLOGY,72(5-6),357-363.
MLA Zhou, Liang,et al."Epigenetic and genetic alterations of the EDNRB gene in nasopharyngeal carcinoma".ONCOLOGY 72.5-6(2007):357-363.
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