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学科主题临床医学
Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells
Guan, Yinghua; Xu, Ming; Liang, Zhangyi; Xu, Ning; Lu, Zhizhen; Han, Qide; Zhang, Youyi; Zhao, Xin Sheng
关键词adrenoceptor single particle tracking living cell
刊名BIOPHYSICAL CHEMISTRY
2007-05-01
DOI10.1016/j.bpc.2007.01.009
127期:3页:149-154
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Chemistry
关键词[WOS]GREEN FLUORESCENT PROTEIN ; HAND-OVER-HAND ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; CELLULAR-LOCALIZATION ; SUBCELLULAR-LOCALIZATION ; MEDIATED INTERNALIZATION ; RECEPTOR SUBTYPES ; LIGAND-BINDING ; EXPRESSION ; INFECTION
英文摘要

The heterogeneous motion of alpha(1B)-adrenoceptor (alpha(1B)-AR) was visualized in living cells with BODIPY-labeled antagonist of AR by single molecule fluorescence microscopy at high spatial resolution. The moving trajectory was reconstructed by precise localization (better than 20 nm) with a least-square fit of a two-dimensional Gaussian point spread function to each single spot. Trajectory analysis revealed two apparent groups of movements: directed motion and hindered motion. The directed motion had speeds higher than 0.1 mu m/s. The histogram of diffusion coefficients of the hindered motion showed distinction between the cell membrane and the cytoplasm: the diffusion coefficient was lower near the cell membrane than in the internal cytoplasm, suggesting that alpha(1B)-AR was located or trapped in different networks, which was consistent with the natural distribution of cytoskeleton in living cells. These results suggested that the heterogeneity in the motion of alpha(1B)-AR in living cell might be associated with different localizations of cell skeleton proteins in the cell, which could provide molecular insight of AR regulation in living cells. (c) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000245829700003
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58274
专题北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Inst Vasc Med, Third Hosp, Beijing 100083, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
3.Peking Univ, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
4.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China
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Guan, Yinghua,Xu, Ming,Liang, Zhangyi,et al. Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells[J]. BIOPHYSICAL CHEMISTRY,2007,127(3):149-154.
APA Guan, Yinghua.,Xu, Ming.,Liang, Zhangyi.,Xu, Ning.,Lu, Zhizhen.,...&Zhao, Xin Sheng.(2007).Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells.BIOPHYSICAL CHEMISTRY,127(3),149-154.
MLA Guan, Yinghua,et al."Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells".BIOPHYSICAL CHEMISTRY 127.3(2007):149-154.
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