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学科主题: 临床医学
题名:
Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells
作者: Guan, Yinghua; Xu, Ming; Liang, Zhangyi; Xu, Ning; Lu, Zhizhen; Han, Qide; Zhang, Youyi; Zhao, Xin Sheng
关键词: adrenoceptor ; single particle tracking ; living cell
刊名: BIOPHYSICAL CHEMISTRY
发表日期: 2007-05-01
DOI: 10.1016/j.bpc.2007.01.009
卷: 127, 期:3, 页:149-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Chemistry
关键词[WOS]: GREEN FLUORESCENT PROTEIN ; HAND-OVER-HAND ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; CELLULAR-LOCALIZATION ; SUBCELLULAR-LOCALIZATION ; MEDIATED INTERNALIZATION ; RECEPTOR SUBTYPES ; LIGAND-BINDING ; EXPRESSION ; INFECTION
英文摘要:

The heterogeneous motion of alpha(1B)-adrenoceptor (alpha(1B)-AR) was visualized in living cells with BODIPY-labeled antagonist of AR by single molecule fluorescence microscopy at high spatial resolution. The moving trajectory was reconstructed by precise localization (better than 20 nm) with a least-square fit of a two-dimensional Gaussian point spread function to each single spot. Trajectory analysis revealed two apparent groups of movements: directed motion and hindered motion. The directed motion had speeds higher than 0.1 mu m/s. The histogram of diffusion coefficients of the hindered motion showed distinction between the cell membrane and the cytoplasm: the diffusion coefficient was lower near the cell membrane than in the internal cytoplasm, suggesting that alpha(1B)-AR was located or trapped in different networks, which was consistent with the natural distribution of cytoskeleton in living cells. These results suggested that the heterogeneity in the motion of alpha(1B)-AR in living cell might be associated with different localizations of cell skeleton proteins in the cell, which could provide molecular insight of AR regulation in living cells. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000245829700003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58274
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Inst Vasc Med, Third Hosp, Beijing 100083, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
3.Peking Univ, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
4.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China

Recommended Citation:
Guan, Yinghua,Xu, Ming,Liang, Zhangyi,et al. Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells[J]. BIOPHYSICAL CHEMISTRY,2007,127(3):149-154.
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