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学科主题: 临床医学
题名:
Pioglitazone suppresses the lipopolysaccharide-induced production of inflammatory factors in mouse macrophages by inactivating NF-kappa B
作者: Ao, Caihui1; Huo, Yong1; Qi, Litong1; Xiong, Zhuowei1; Xue, Lin1; Qi, Yongfen2
关键词: inflammation ; lipopolysaccharide ; macrophage ; pioglitazone ; secretory phospholipase A2 ; tumour necrosis factor alpha (TNF-alpha)
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2010-07-01
DOI: 10.1042/CBI20090005
卷: 34, 期:7, 页:723-730
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: TUMOR-NECROSIS-FACTOR ; SECRETORY PHOSPHOLIPASE A(2) ; CORONARY-ARTERY-DISEASE ; LOW-DENSITY-LIPOPROTEIN ; FACTOR-ALPHA PRODUCTION ; FOAM CELL-FORMATION ; PPAR-GAMMA ; ROSIGLITAZONE TREATMENT ; GENE-EXPRESSION ; IN-VITRO
英文摘要:

TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis factor alpha), sPLA2-V and -X (groups V and X sPLA2) in RAW 264.7 macrophages. TNF-alpha, sPLA2-V and -X mRNA and protein expression were determined by RT-PCR (reverse transcriptase-PCR) and Western blot analysis, respectively. The activity of NF-kappa B (nuclear factor kappa B) was determined by Western blot and confocal microscopy. LPS induced TNF-alpha, sPLA2-V and sPLA2-X mRNA and protein expression. Pretreatment with 10 mu mol/l pioglitazone significantly suppressed LPS-induced TNF-alpha, sPLA2-V and sPLA2-X mRNA and protein expression. LPS induced NF-kappa B expression and translocation in the nucleus, but the inductive effects were inhibited by pioglitazone. Our findings indicate that pioglitazone inhibits production of inflammatory factors induced by LPS in murine macrophage cells by inactivating NF-kappa B. Pioglitazone appears to play an anti-inflammatory role in the atherosclerotic process.

语种: 英语
所属项目编号: 30470347
项目资助者: National Natural Science Fund of China
WOS记录号: WOS:000280039400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58286
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China

Recommended Citation:
Ao, Caihui,Huo, Yong,Qi, Litong,et al. Pioglitazone suppresses the lipopolysaccharide-induced production of inflammatory factors in mouse macrophages by inactivating NF-kappa B[J]. CELL BIOLOGY INTERNATIONAL,2010,34(7):723-730.
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