IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Low Molecular Weight Apple Polysaccharides Induced Cell Cycle Arrest in Colorectal Tumor
Li, Yuhua1,2; Mei, Lin3; Niu, Yinbo4; Sun, Yang2; Huang, Haitao2; Li, Qian2; Kong, Xianghe4; Liu, Li2; Li, Zhiquan1; Mei, Qibing2,4
刊名NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
2012-04-01
DOI10.1080/01635581.2012.658951
64期:3页:439-463
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Nutrition & Dietetics
资助者National Program on Key Basic Research Project of China (973 Program) ; Technology Gallery of Shaanxi Province ; National Program on Key Basic Research Project of China (973 Program) ; Technology Gallery of Shaanxi Province
研究领域[WOS]Oncology ; Nutrition & Dietetics
关键词[WOS]COLON-CANCER ; ANTIINFLAMMATORY DRUGS ; CONTINUOUS-INFUSION ; JUICE EXTRACTS ; INHIBIT GROWTH ; PREVENTION ; CARCINOMA ; APOPTOSIS ; FLUOROURACIL ; STATISTICS
英文摘要

Dietary components play an important role in cancer prevention. Many ingredients from apples have been proven to have anti-tumor potency. We thus made low molecular weight apple polysaccharides (LMWAP) and evaluated the effects of it on colorectal cancer (CRC). The effects of LMWAP on human colon carcinoma cells (HT-29) were evaluated using a microarray. Then, cell-cycle distribution was measured by flow cytometric analysis. A colitis-associated colorectal cancer mouse model was used to assess the effect of LMWAP on in vivo CRC prevention. Treatment of HT-29 cells with LMWAP resulted in 333 genes expression over cutoff values (>= 2-fold). Further analysis demonstrated that pathways of cell cycle were mainly influenced. At the concentrations from 0.001 to 0.1 mg/mL, LMWAP induced a G(0)/G(1) phase block in HT-29 cells in a dose-dependent way. In vivo studies revealed that administration of LMWAP could protect ICR mice against CRC effectively. The results of Western blot suggested LMWAP induced cell-cycle arrest in a p53 independent manner. These data indicate that LMWAP could inhibit the development of CRC through affecting cell cycle, and it has potential for clinical prevention for colon cancer.

语种英语
所属项目编号2010CB 535002 ; 2010ZDKG-47
资助者National Program on Key Basic Research Project of China (973 Program) ; Technology Gallery of Shaanxi Province ; National Program on Key Basic Research Project of China (973 Program) ; Technology Gallery of Shaanxi Province
WOS记录号WOS:000303702600011
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58290
Collection北京大学第三临床医学院_心血管内科
作者单位1.422 Hosp PLA, Dept Pharm, Zhanjiang 524005, Guangdong, Peoples R China
2.Fourth Mil Med Univ, Sch Pharm, Dept Pharmacol,State Adm Tradit Chinese Med, Key Lab Gastrointestinal Pharmacol Chinese Mat Me, Xian 710032, Shaanxi, Peoples R China
3.Beijing Univ, Dept Cardiol, Subsidiary Hosp 3, Beijing 100871, Peoples R China
4.Northwestern Polytech Univ, Sch Life Sci, Xian, Shaanxi, Peoples R China
Recommended Citation
GB/T 7714
Li, Yuhua,Mei, Lin,Niu, Yinbo,et al. Low Molecular Weight Apple Polysaccharides Induced Cell Cycle Arrest in Colorectal Tumor[J]. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL,2012,64(3):439-463.
APA Li, Yuhua.,Mei, Lin.,Niu, Yinbo.,Sun, Yang.,Huang, Haitao.,...&Mei, Qibing.(2012).Low Molecular Weight Apple Polysaccharides Induced Cell Cycle Arrest in Colorectal Tumor.NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL,64(3),439-463.
MLA Li, Yuhua,et al."Low Molecular Weight Apple Polysaccharides Induced Cell Cycle Arrest in Colorectal Tumor".NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL 64.3(2012):439-463.
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