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Negative regulation of monocyte chemoattractant protein-1 gene expression by a mouse estrogen-enhanced transcript
Xie, LP; Fu, WX; Jin, C; Dong, XY; Chen, WF
关键词chemokine estrogen estrogen-responsive element NF-kappa B thymus stromal cell
刊名EUROPEAN JOURNAL OF IMMUNOLOGY
2002-10-01
32期:10页:2837-2846
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]DIFFERENTIAL DISPLAY ; CHEMOKINE ; CLONING ; CELLS ; BETA
英文摘要

A novel gene containing two typical estrogen responsive elements (ERE) was cloned from MTEC1 cells, a mouse thymus epithelial cell line that produces constitutively many chemokines. This gene is a homologue of the rat estrogen-enhanced transcript (EET) gene, and is called the mEET gene. mEET protein is expressed in cytoplasm. Addition of 17 [P-estradiol (E2) to the MTEC1 cell cultures enhanced mEET mRNA expression and, meanwhile, significantly inhibited monocyte chemoattractant protein-1 (MCP-1) production. To analyze the functional links between the expression of mEET and of MCP-1, we transfected MTEC1 cells with ERE-deleted antisense- or sense-mEET complementary (c)DNA construct and activated the transfected mEET cDNA in stable MTEC1 transfectants with doxycycline (Dox). Dox-induced activation of the mEET gene profoundly inhibited MCP-1 expression at both mRNA and protein levels and alleviated its chemotactic activity. Conversely, inactivation of the mEET gene substantially augmented MCP-1 expression. Activation of the mEET gene markedly attenuated activity of nuclear NF-kappaB. In summary, we have first demonstrated that estrogen-imposed inhibition of MCP-1 expression occurs through the activation of the mEET gene, its product suppresses nuclear NF-kappaB and negatively regulates MCP-1 gene activation.

语种英语
WOS记录号WOS:000178746600015
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58292
专题基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
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Xie, LP,Fu, WX,Jin, C,et al. Negative regulation of monocyte chemoattractant protein-1 gene expression by a mouse estrogen-enhanced transcript[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2002,32(10):2837-2846.
APA Xie, LP,Fu, WX,Jin, C,Dong, XY,&Chen, WF.(2002).Negative regulation of monocyte chemoattractant protein-1 gene expression by a mouse estrogen-enhanced transcript.EUROPEAN JOURNAL OF IMMUNOLOGY,32(10),2837-2846.
MLA Xie, LP,et al."Negative regulation of monocyte chemoattractant protein-1 gene expression by a mouse estrogen-enhanced transcript".EUROPEAN JOURNAL OF IMMUNOLOGY 32.10(2002):2837-2846.
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