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IR@PKUHSC  > 北京大学药学院  > 天然药物学系  > 期刊论文
学科主题: 药学
题名:
Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System
作者: Niu, Yaru; Yin, Liangliang; Luo, Shiheng; Dong, Jing2; Wang, Hong; Hashi, Yuki2; Chen, Shizhong1
关键词: HPLC-DAD-ESI ; MSn ; post-column UV-derivatisation ; anti-oxidants ; Flos Chrysanthemi
刊名: PHYTOCHEMICAL ANALYSIS
发表日期: 2013
DOI: 10.1002/pca.2381
卷: 24, 期:1, 页:59-68
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
关键词[WOS]: MASS-SPECTROMETRY ; LC-MSN ; CHLOROGENIC ACIDS ; EXTRACTS ; FLOWERS ; CONSTITUENTS ; DERIVATIVES ; FLAVONOIDS ; COMPOSITAE
英文摘要:

Introduction Flos Chrysanthemi (Jiju) is a traditional Chinese medicine (TCM) that is known to have anti-oxidant activity; in this study, on-line HPLCDADESI/MSn and HPLCDADDPPH methods have been developed for rapidly screening and identifying free-radical scavengers in Jiju extract. Objective To develop an efficient method for the simultaneous identification and detection of the anti-oxidant components in Flos Chrysanthemi (Jiju). Methodology A concentrated methanol extract of Flos Chrysanthemi from Jiaxiang County (Jiju) was first separated into phases soluble in water, petroleum ether and n-butanol. The off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging method was then used to evaluate the anti-oxidant activity of each phase in vitro. The results showed that the n-butanol extract had the highest anti-oxidant activity, and its anti-oxidant compounds were analysed by HPLCDADESI/MSn and HPLC coupled with a post-column derivatisation (PCD) system supplied with DPPH, aluminium chloride or sodium acetate solutions. Results A total of 17 compounds were separated and identified, three of which were identified in Jiju for the first time, and seven active compounds serve as the chemical basis of the anti-oxidant efficacy of Jiju. Conclusion The methods described here allow rapid separation and convenient identification of the multiple constituents in Jiju, and may be applied to other complex natural matrices. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2006CB504707
项目资助者: national 973 research project ; Shimadzu International Trading (Shanghai) Co. Limited
WOS记录号: WOS:000312141800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58308
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai, Beijing Off, Beijing 100020, Peoples R China

Recommended Citation:
Niu, Yaru,Yin, Liangliang,Luo, Shiheng,et al. Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System[J]. PHYTOCHEMICAL ANALYSIS,2013,24(1):59-68.
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