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Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System
Niu, Yaru; Yin, Liangliang; Luo, Shiheng; Dong, Jing2; Wang, Hong; Hashi, Yuki2; Chen, Shizhong1
关键词HPLC-DAD-ESI MSn post-column UV-derivatisation anti-oxidants Flos Chrysanthemi
刊名PHYTOCHEMICAL ANALYSIS
2013
DOI10.1002/pca.2381
24期:1页:59-68
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; LC-MSN ; CHLOROGENIC ACIDS ; EXTRACTS ; FLOWERS ; CONSTITUENTS ; DERIVATIVES ; FLAVONOIDS ; COMPOSITAE
英文摘要

Introduction Flos Chrysanthemi (Jiju) is a traditional Chinese medicine (TCM) that is known to have anti-oxidant activity; in this study, on-line HPLCDADESI/MSn and HPLCDADDPPH methods have been developed for rapidly screening and identifying free-radical scavengers in Jiju extract. Objective To develop an efficient method for the simultaneous identification and detection of the anti-oxidant components in Flos Chrysanthemi (Jiju). Methodology A concentrated methanol extract of Flos Chrysanthemi from Jiaxiang County (Jiju) was first separated into phases soluble in water, petroleum ether and n-butanol. The off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging method was then used to evaluate the anti-oxidant activity of each phase in vitro. The results showed that the n-butanol extract had the highest anti-oxidant activity, and its anti-oxidant compounds were analysed by HPLCDADESI/MSn and HPLC coupled with a post-column derivatisation (PCD) system supplied with DPPH, aluminium chloride or sodium acetate solutions. Results A total of 17 compounds were separated and identified, three of which were identified in Jiju for the first time, and seven active compounds serve as the chemical basis of the anti-oxidant efficacy of Jiju. Conclusion The methods described here allow rapid separation and convenient identification of the multiple constituents in Jiju, and may be applied to other complex natural matrices. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000312141800007
项目编号2006CB504707
资助机构national 973 research project ; Shimadzu International Trading (Shanghai) Co. Limited
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58308
专题北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai, Beijing Off, Beijing 100020, Peoples R China
推荐引用方式
GB/T 7714
Niu, Yaru,Yin, Liangliang,Luo, Shiheng,et al. Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System[J]. PHYTOCHEMICAL ANALYSIS,2013,24(1):59-68.
APA Niu, Yaru.,Yin, Liangliang.,Luo, Shiheng.,Dong, Jing.,Wang, Hong.,...&Chen, Shizhong.(2013).Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System.PHYTOCHEMICAL ANALYSIS,24(1),59-68.
MLA Niu, Yaru,et al."Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MSn and HPLC Coupled with a Post-column Derivatisation System".PHYTOCHEMICAL ANALYSIS 24.1(2013):59-68.
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