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学科主题临床医学
Identification of proteins that interact with alpha A-crystallin using a human proteome microarray
Fan, Qi1; Huang, Lv-Zhen2; Zhu, Xiang-Jia1; Zhang, Ke-Ke1; Ye, Hong-Fei1; Luo, Yi1; Sun, Xing-Huai1; Zhou, Peng3; Lu, Yi1
刊名MOLECULAR VISION
2014-01-14
20页:117-124
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
资助者National Natural Science Foundation of China (NSFC) ; Doctoral Program of Higher Education of China (RFDP) ; Ministry of Public Health Research ; Fudan University ; National Natural Science Foundation of China (NSFC) ; Doctoral Program of Higher Education of China (RFDP) ; Ministry of Public Health Research ; Fudan University
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]LENS EPITHELIAL-CELLS ; B-CRYSTALLIN ; ACTIN ; MOUSE ; TRANSPARENCY ; STABILIZES ; CATARACT ; PREVENTS ; BINDING ; TISSUES
英文摘要

Purpose: To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray.

Methods: The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. The probes were considered detectable when the signal to noise ratio (SNR) was over 1.2. The identified proteins were subjected to subsequent bioinformatics analysis using the DAVID database.

Results: The HuProt microarray results showed that the signals of 343 proteins were higher in the recombinant CRYAA group than in the control group. The SNR of 127 proteins was >= 1.2. The SNR of the following eight proteins was > 3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). The bioinformatics analysis revealed 127 proteins associated with phosphoproteins, alternative splicing, acetylation, DNA binding, the nuclear lumen, ribonucleotide binding, the cell cycle, WD40 repeats, protein transport, transcription factor activity, GTP binding, and cellular response to stress. Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. The protein-protein interactions may help CRYAA carry out multifaceted functions.

Conclusions: One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. The advent of microarray analysis enables a better understanding of the functions of CRYAA as a molecular chaperone.

语种英语
所属项目编号81200669 ; 81270989 ; 81100653 ; 20120071120089 ; 201302015
资助者National Natural Science Foundation of China (NSFC) ; Doctoral Program of Higher Education of China (RFDP) ; Ministry of Public Health Research ; Fudan University ; National Natural Science Foundation of China (NSFC) ; Doctoral Program of Higher Education of China (RFDP) ; Ministry of Public Health Research ; Fudan University
WOS记录号WOS:000331825400002
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58311
专题北京大学第二临床医学院_眼科
作者单位1.Pkwy Hlth Hong Qiao Med Ctr, Dept Ophthalmol, Shanghai, Peoples R China
2.Fudan Univ, Eye & ENT Hosp, Dept Ophthalmol, Shanghai 200031, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China
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GB/T 7714
Fan, Qi,Huang, Lv-Zhen,Zhu, Xiang-Jia,et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray[J]. MOLECULAR VISION,2014,20:117-124.
APA Fan, Qi.,Huang, Lv-Zhen.,Zhu, Xiang-Jia.,Zhang, Ke-Ke.,Ye, Hong-Fei.,...&Lu, Yi.(2014).Identification of proteins that interact with alpha A-crystallin using a human proteome microarray.MOLECULAR VISION,20,117-124.
MLA Fan, Qi,et al."Identification of proteins that interact with alpha A-crystallin using a human proteome microarray".MOLECULAR VISION 20(2014):117-124.
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