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学科主题基础医学
A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study
Song, Xiao-Li1; Zhang, Qing-Ying2; Wang, Zhe-Ming1; Fu, Hong-Zheng3; Qian, Rui-Qin1
关键词kirenol RP-HPLC SPE pharmacokinetic
刊名BIOMEDICAL CHROMATOGRAPHY
2011-05-01
DOI10.1002/bmc.1481
25期:5页:542-546
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SIEGESBECKIA-PUBESCENS
英文摘要

A rapid and simple reverse-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for the quantification of kirenol in rat plasma after oral administration. Kirenol and darutoside (internal standard, IS) were extracted from rat plasma using Cleanert (TM) C-18 solid-phase extraction (SPE) cartridge. Analysis of the extraction was performed on a Thermo ODS-2 Hypersil C-18 reversed-phase column with a gradient eluent composed of acetonitrile and 0.1% phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 215 nm. The calibration curve was linear over the range of 9.756-133.333 mu g/mL (r(2) = 0.9991) in rat plasma. The lower limits of detection and quantification were 2.857 and 9.756 mu g/mL, respectively. The intra-and inter-day precisions (relative standard deviation, RSD) were between 2.24 and 4.46%, with accuracies ranging from 91.80 to 102.74%. The extraction recovery ranged from 98.16 to 107.62% with RSD less than 4.81%. Stability studies showed that kirenol was stable in preparation and analytical process. The present method was successfully applied to the pharmacokinetic study of kirenol in male Sprague-Dawley rats after oral administration at a dose of 50 mg/kg. Copyright (C) 2010 JohnWiley & Sons, Ltd.

语种英语
WOS记录号WOS:000289214100003
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58330
专题北京大学药学院_天然药物学系
北京大学基础医学院
北京大学药学院
作者单位1.Peking Univ, Dept Integrated Chinese Tradit Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Song, Xiao-Li,Zhang, Qing-Ying,Wang, Zhe-Ming,et al. A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2011,25(5):542-546.
APA Song, Xiao-Li,Zhang, Qing-Ying,Wang, Zhe-Ming,Fu, Hong-Zheng,&Qian, Rui-Qin.(2011).A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,25(5),542-546.
MLA Song, Xiao-Li,et al."A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 25.5(2011):542-546.
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