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学科主题: 基础医学
题名:
A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study
作者: Song, Xiao-Li1; Zhang, Qing-Ying2; Wang, Zhe-Ming1; Fu, Hong-Zheng3; Qian, Rui-Qin1
关键词: kirenol ; RP-HPLC ; SPE ; pharmacokinetic
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2011-05-01
DOI: 10.1002/bmc.1481
卷: 25, 期:5, 页:542-546
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SIEGESBECKIA-PUBESCENS
英文摘要:

A rapid and simple reverse-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for the quantification of kirenol in rat plasma after oral administration. Kirenol and darutoside (internal standard, IS) were extracted from rat plasma using Cleanert (TM) C-18 solid-phase extraction (SPE) cartridge. Analysis of the extraction was performed on a Thermo ODS-2 Hypersil C-18 reversed-phase column with a gradient eluent composed of acetonitrile and 0.1% phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 215 nm. The calibration curve was linear over the range of 9.756-133.333 mu g/mL (r(2) = 0.9991) in rat plasma. The lower limits of detection and quantification were 2.857 and 9.756 mu g/mL, respectively. The intra-and inter-day precisions (relative standard deviation, RSD) were between 2.24 and 4.46%, with accuracies ranging from 91.80 to 102.74%. The extraction recovery ranged from 98.16 to 107.62% with RSD less than 4.81%. Stability studies showed that kirenol was stable in preparation and analytical process. The present method was successfully applied to the pharmacokinetic study of kirenol in male Sprague-Dawley rats after oral administration at a dose of 50 mg/kg. Copyright (C) 2010 JohnWiley & Sons, Ltd.

语种: 英语
所属项目编号: 7072043
项目资助者: Natural Science Foundation of Beijing, China
WOS记录号: WOS:000289214100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58330
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Integrated Chinese Tradit Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Song, Xiao-Li,Zhang, Qing-Ying,Wang, Zhe-Ming,et al. A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2011,25(5):542-546.
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