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Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma
Zhang, You-Bo; Yang, Xiu-Wei
关键词Column liquid chromatography Pharmacokinetic studies p-Hydroxyphenethyl anisate in rat plasma Notopterygium Radix
刊名CHROMATOGRAPHIA
2009-08-01
DOI10.1365/s10337-009-1159-z
70期:3-4页:591-595
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHINESE CRUDE DRUG ; NOTOPTERYGIUM-INCISUM ; UNDERGROUND PARTS ; QIANG-HUO ; FORBESII
英文摘要

A rapid, sensitive and specific reversed-phase liquid chromatographic method was developed and validated for the quantification of p-hydroxyphenethyl anisate (HPA), which is one of the main constituents of Notopterygium Radix (underground parts of Notopterygium incisum and N. forbesii), in rat plasma, and study its pharmacokinetics after the intravenous administration of 40 mg kg(-1) HPA to rats. The method involves a plasma clear-up step using liquid-liquid extraction by ethyl acetate, followed by RP-LC separation and detection. Separation of HPA was performed on an analytical Diamonsil ODS C(18) column equipped with a Dikma ODS C(18) EasyGuard column using a mobile phase consisting of MeOH-H(2)O (75:25, v/v) at a flow-rate of 1.0 mL min(-1). The UV detection was performed at a wavelength of 256 nm. The linear calibration curves were obtained in the concentration range of 0.05-5.0 mu g mL(-1) (r = 0.9992, n = 5) in rat plasma with the lower limit of detection of 0.01 mu g mL(-1) and the lower limit of quantification of 0.04 mu g mL(-1), and the extraction recovery of HPA was calculated to be the range of 82.01-86.66%. The intra- and inter-day precisions in terms of % relative standard deviation were lower than 2.33 and 3.99% in rat plasma, respectively, with accuracies ranging from 91.22 to 110.5%. The developed method was suitable for the determination and pharmacokinetic study of HPA in rat plasma.

语种英语
所属项目编号30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2006BAI06A01-02
资助者National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program
WOS记录号WOS:000268995600034
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58331
专题北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, You-Bo,Yang, Xiu-Wei. Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma[J]. CHROMATOGRAPHIA,2009,70(3-4):591-595.
APA Zhang, You-Bo,&Yang, Xiu-Wei.(2009).Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma.CHROMATOGRAPHIA,70(3-4),591-595.
MLA Zhang, You-Bo,et al."Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma".CHROMATOGRAPHIA 70.3-4(2009):591-595.
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