北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma
作者: Zhang, You-Bo; Yang, Xiu-Wei
关键词: Column liquid chromatography ; Pharmacokinetic studies ; p-Hydroxyphenethyl anisate in rat plasma ; Notopterygium Radix
刊名: CHROMATOGRAPHIA
发表日期: 2009-08-01
DOI: 10.1365/s10337-009-1159-z
卷: 70, 期:3-4, 页:591-595
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CHINESE CRUDE DRUG ; NOTOPTERYGIUM-INCISUM ; UNDERGROUND PARTS ; QIANG-HUO ; FORBESII
英文摘要:

A rapid, sensitive and specific reversed-phase liquid chromatographic method was developed and validated for the quantification of p-hydroxyphenethyl anisate (HPA), which is one of the main constituents of Notopterygium Radix (underground parts of Notopterygium incisum and N. forbesii), in rat plasma, and study its pharmacokinetics after the intravenous administration of 40 mg kg(-1) HPA to rats. The method involves a plasma clear-up step using liquid-liquid extraction by ethyl acetate, followed by RP-LC separation and detection. Separation of HPA was performed on an analytical Diamonsil ODS C(18) column equipped with a Dikma ODS C(18) EasyGuard column using a mobile phase consisting of MeOH-H(2)O (75:25, v/v) at a flow-rate of 1.0 mL min(-1). The UV detection was performed at a wavelength of 256 nm. The linear calibration curves were obtained in the concentration range of 0.05-5.0 mu g mL(-1) (r = 0.9992, n = 5) in rat plasma with the lower limit of detection of 0.01 mu g mL(-1) and the lower limit of quantification of 0.04 mu g mL(-1), and the extraction recovery of HPA was calculated to be the range of 82.01-86.66%. The intra- and inter-day precisions in terms of % relative standard deviation were lower than 2.33 and 3.99% in rat plasma, respectively, with accuracies ranging from 91.22 to 110.5%. The developed method was suitable for the determination and pharmacokinetic study of HPA in rat plasma.

语种: 英语
所属项目编号: 30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2006BAI06A01-02
项目资助者: National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program
WOS记录号: WOS:000268995600034
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58331
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, You-Bo,Yang, Xiu-Wei. Rapid and Sensitive RP-LC Method for the Quantification and Pharmacokinetic Study of p-Hydroxyphenethyl Anisate in Rat Plasma[J]. CHROMATOGRAPHIA,2009,70(3-4):591-595.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, You-Bo]'s Articles
[Yang, Xiu-Wei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, You-Bo]‘s Articles
[Yang, Xiu-Wei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace