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学科主题: 临床医学
题名:
Identification of genes specifically expressed in the accumulated visceral adipose tissue of OLETF rats
作者: Hida, K; Wada, J; Zhang, H; Hiragushi, K; Tsuchiyama, Y; Shikata, K; Makino, H
关键词: obesity ; OLETF rats ; visceral adipose tissue ; representational difference analysis ; PCR-based subtraction method ; molecular cloning
刊名: JOURNAL OF LIPID RESEARCH
发表日期: 2000-10-01
卷: 41, 期:10, 页:1615-1622
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: EPITHELIAL MEMBRANE PROTEIN-1 ; TUMOR-NECROSIS-FACTOR ; INSULIN-RESISTANCE ; BINDING-PROTEIN ; MOUSE KIDNEY ; IN-VIVO ; OBESITY ; DISEASE ; ANGIOGENESIS ; TROGLITAZONE
英文摘要:

The Otsuka Long-Evans Tokushima fatty (OLETF) rat is an animal model of type 2 diabetes, characterized by abdominal obesity, insulin resistance, hypertension, and dyslipidemia. To elucidate the underlying molecular mechanism of obesity and its related complications, we used representational difference analysis and identified the genes more abundantly and specifically expressed in the visceral adipose tissue (VAT) of obese OLETF rats compared with the diabetes-resistant counterpart, that is, Long Evans Tokushima Otsuka (LETO) rats. By Northern blot analysis, we con firmed the differential expression of 13 genes, including 3 novel genes. The upregulated expression of well-characterized lipid metabolic enzymes, such as lipoprotein lipase, phosphoenolpyruvate carboxykinase, and cholesterol esterase, were observed in VAT of OLETF rats. We demonstrated the differential expression of secreted proteins in VAT of OLETF rats, such as thrombospondin 1 and contrapsin-like protease inhibitor. In contrast to lipid enzymes, the secreted proteins revealed exclusive mRNA expression and they were not detected in VAT of LETO rats. Furthermore, the novel genes OL-16 and OL-64 were also expressed specifically in VAT of OLETF rats and were absent in that of LETO rats and other tissues, including subdermal and brown adipose tissues. The C-terminal partial amino acid sequence of OL-64 revealed that it showed similar to 40% homology with alpha(1)-antitrypsin and it seemed to be a new member of the serine proteinase inhibitor (SERPIN) gem family. VAT of OLETF rats had a unique gem expression profile, and the accumulated VAT-specific known and novel secreted proteins may play a role(s) in the pathogenesis of obesity and its related complications.

语种: 英语
WOS记录号: WOS:000089667800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58344
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Okayama Univ, Sch Med, Dept Med 2, Okayama 7008558, Japan
2.Beijing Med Univ, Teaching Hosp 1, Dept Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Hida, K,Wada, J,Zhang, H,et al. Identification of genes specifically expressed in the accumulated visceral adipose tissue of OLETF rats[J]. JOURNAL OF LIPID RESEARCH,2000,41(10):1615-1622.
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