IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women
Zhang, Rui1; Daroczy, Katalin2; Xiao, Bingbing1; Yu, Li1; Chen, Rui1; Liao, Qinping1
刊名JOURNAL OF MEDICAL MICROBIOLOGY
2012-05-01
DOI10.1099/jmm.0.038687-0
61期:5页:729-739
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]GRADIENT GEL-ELECTROPHORESIS ; PLACEBO-CONTROLLED TRIAL ; LACTIC-ACID BACTERIA ; CULTIVATION-INDEPENDENT METHODS ; HORMONE REPLACEMENT THERAPY ; ORAL METRONIDAZOLE THERAPY ; RHAMNOSUS GR-1 ; GARDNERELLA-VAGINALIS ; MICROBIAL COMMUNITIES ; ATOPOBIUM-VAGINAE
英文摘要

The present study aimed to investigate the prevalence and quantity of Lactobacillus species in the vaginas of healthy Chinese women. Vaginal samples from 92 fertile and 22 postmenopausal healthy Chinese women were analysed using a 16S rRNA gene clone library and species-specific PCR followed by sequencing and real-time PCR. A total of 13 different Lactobacillus species were detected. Species-specific PCR showed that 3% of the fertile women were colonized by one species of Lactobacillus and 97% were colonized by two or more species. Among the postmenopausal women, 91% were colonized by one species of Lactobacillus and 9% were colonized by two species. In the fertile women, L. iners (82.61%), L. crispatus (70.65%) and L. gasseri (67.39%) were more prevalent than L. jensenii (40.22%), L. acidophilus (39.13%), L. brevis (23.91%), L. plantarum (5.43%), L. johnsonii (3.26%), L. fermentum (2.17%), L. salivarius (2.17%), L. rhamnosus (1.09%), L. reuteri (1.09%) and L. paracasei (1.09%); L. delbrueckii was not detected. In the postmenopausal women, L. fermentum, L. rhamnosus, L. reuteri and L. delbrueckii were not detected, and the other 10 species were detected in just a few samples. The prevalence of these species according to the clone library differed from the prevalence indicated by the species-specific PCR. According to the semiquantitative analysis, the total Lactobacillus DNA concentrations were higher in fertile women than in postmenopausal women. Sixty-one per cent of the fertile women were predominantly colonized by L. iners, 35% by L. crispatus, and 2% by L. gasseri. Associations between pairs of Lactobacillus species in fertile women were significant (P<0.05) between the following pairs: L. iners and L. gasseri, L. iners and L. jensenii, L. iners and L. acidophilus, L. gasseri and L. acidophilus, and L. gasseri and L. jensenii. In conclusion, this study provided detailed information on Lactobacillus species colonizing the vaginas of healthy Chinese fertile and postmenopausal women. The study also showed that the diversity of Lactobacillus species in fertile women was higher than in postmenopausal women. According to our study, different techniques, such as species-specific PCR and comparison against a 16S rRNA gene clone library, resulted in different findings regarding species prevalence. These findings highlight the importance of standardization of techniques used for evaluation of bacterial communities. According to our findings regarding species associations, L. iners and L. gasseri may have influences on colonization and proliferation of other vaginal Lactobacillus species.

语种英语
WOS记录号WOS:000304217700017
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58352
专题北京大学第一临床医学院
作者单位1.Ellen AB, S-18233 Danderyd, Sweden
2.Peking Univ, Dept Obstet & Gynecol, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Rui,Daroczy, Katalin,Xiao, Bingbing,et al. Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2012,61(5):729-739.
APA Zhang, Rui,Daroczy, Katalin,Xiao, Bingbing,Yu, Li,Chen, Rui,&Liao, Qinping.(2012).Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women.JOURNAL OF MEDICAL MICROBIOLOGY,61(5),729-739.
MLA Zhang, Rui,et al."Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women".JOURNAL OF MEDICAL MICROBIOLOGY 61.5(2012):729-739.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Rui]的文章
[Daroczy, Katalin]的文章
[Xiao, Bingbing]的文章
百度学术
百度学术中相似的文章
[Zhang, Rui]的文章
[Daroczy, Katalin]的文章
[Xiao, Bingbing]的文章
必应学术
必应学术中相似的文章
[Zhang, Rui]的文章
[Daroczy, Katalin]的文章
[Xiao, Bingbing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。