IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder
Han, Yonghua1,2; Liu, Yuchen1,2; Gui, Yaoting1,2; Cai, Zhiming1,2,3
关键词microRNA neoplasm targeting
刊名JOURNAL OF SURGICAL ONCOLOGY
2013-02-01
DOI10.1002/jso.23214
107期:2页:201-205
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Surgery
研究领域[WOS]Oncology ; Surgery
关键词[WOS]CANCER-CELLS ; EXPRESSION ; OVEREXPRESSION ; MICRORNAS ; PHENOTYPE
英文摘要

Background and Objectives MicroRNAs (miRNAs) are aberrantly expressed in cancers. Dicer, Drosha, and Exportin 5 are essential for miRNA processing. In this study, the expression patterns of Dicer, Drosha, and Exportin 5 and the cell proliferation inhibition and apoptosis induced by silencing these genes in urothelial carcinoma of the bladder were determined. Methods The expression levels of Dicer, Drosha, and Exportin 5 were determined using Real-Time qPCR in 40 patients with urothelial carcinoma of the bladder. Bladder urothelial carcinoma T24 and 5637 cells were transfected with Dicer, Drosha, or Exportin 5 siRNA or negative control siRNA. Cell proliferation was determined using MTT assay. Apoptosis was evaluated using ELISA assay. Results All the three genes were up-regulated in bladder urothelial carcinoma compared to matched normal urothelium. Dicer, Drosha, and Exportin 5 expression levels were higher in high grade carcinomas than that in low grade carcinomas. Invasive carcinomas had higher expression levels than non-invasive carcinomas. Silencing Dicer, Drosha, or Exportin 5 induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma T24 and 5637 cells. Conclusions Dicer, Drosha, and Exportin 5 are promising biomarkers and therapeutic targets for urothelial carcinoma of the bladder. J. Surg. Oncol. 2013;107:201205. (c) 2012 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000313708600016
项目编号2009AA022707 ; CXB200903090055A ; CXB201005250016A ; CXC201005260001A
资助机构National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58368
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen 518036, Peoples R China
2.Shenzhen PKU HKUST Med Ctr, Inst Urol, Shenzhen, Peoples R China
3.Shenzhen Second Peoples Hosp, Dept Urol, Shenzhen, Peoples R China
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Han, Yonghua,Liu, Yuchen,Gui, Yaoting,et al. Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder[J]. JOURNAL OF SURGICAL ONCOLOGY,2013,107(2):201-205.
APA Han, Yonghua,Liu, Yuchen,Gui, Yaoting,&Cai, Zhiming.(2013).Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder.JOURNAL OF SURGICAL ONCOLOGY,107(2),201-205.
MLA Han, Yonghua,et al."Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder".JOURNAL OF SURGICAL ONCOLOGY 107.2(2013):201-205.
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