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学科主题: 临床医学
题名:
Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder
作者: Han, Yonghua1,2; Liu, Yuchen1,2; Gui, Yaoting1,2; Cai, Zhiming1,2,3
关键词: microRNA ; neoplasm ; targeting
刊名: JOURNAL OF SURGICAL ONCOLOGY
发表日期: 2013-02-01
DOI: 10.1002/jso.23214
卷: 107, 期:2, 页:201-205
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Surgery
研究领域[WOS]: Oncology ; Surgery
关键词[WOS]: CANCER-CELLS ; EXPRESSION ; OVEREXPRESSION ; MICRORNAS ; PHENOTYPE
英文摘要:

Background and Objectives MicroRNAs (miRNAs) are aberrantly expressed in cancers. Dicer, Drosha, and Exportin 5 are essential for miRNA processing. In this study, the expression patterns of Dicer, Drosha, and Exportin 5 and the cell proliferation inhibition and apoptosis induced by silencing these genes in urothelial carcinoma of the bladder were determined. Methods The expression levels of Dicer, Drosha, and Exportin 5 were determined using Real-Time qPCR in 40 patients with urothelial carcinoma of the bladder. Bladder urothelial carcinoma T24 and 5637 cells were transfected with Dicer, Drosha, or Exportin 5 siRNA or negative control siRNA. Cell proliferation was determined using MTT assay. Apoptosis was evaluated using ELISA assay. Results All the three genes were up-regulated in bladder urothelial carcinoma compared to matched normal urothelium. Dicer, Drosha, and Exportin 5 expression levels were higher in high grade carcinomas than that in low grade carcinomas. Invasive carcinomas had higher expression levels than non-invasive carcinomas. Silencing Dicer, Drosha, or Exportin 5 induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma T24 and 5637 cells. Conclusions Dicer, Drosha, and Exportin 5 are promising biomarkers and therapeutic targets for urothelial carcinoma of the bladder. J. Surg. Oncol. 2013;107:201205. (c) 2012 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 2009AA022707 ; CXB200903090055A ; CXB201005250016A ; CXC201005260001A
项目资助者: National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen
WOS记录号: WOS:000313708600016
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58368
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen 518036, Peoples R China
2.Shenzhen PKU HKUST Med Ctr, Inst Urol, Shenzhen, Peoples R China
3.Shenzhen Second Peoples Hosp, Dept Urol, Shenzhen, Peoples R China

Recommended Citation:
Han, Yonghua,Liu, Yuchen,Gui, Yaoting,et al. Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder[J]. JOURNAL OF SURGICAL ONCOLOGY,2013,107(2):201-205.
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