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Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1
Hyun, JW; Choi, JY; Zeng, HH; Lee, YS; Kim, HS; Yoon, SH; Chung, MH
关键词KG-1 8-hydroxyguanine hOGG1 mutation cytotoxicity
刊名ONCOGENE
2000-09-14
19期:39页:4476-4479
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]REMOVES 8-HYDROXYGUANINE RESIDUES ; OXIDATIVE DNA-DAMAGE ; ESCHERICHIA-COLI ; ENDONUCLEASE ACTIVITY ; OGG1 GENE ; GLYCOSYLASE ; CLONING ; REPAIR ; EXCISION ; PROTEIN
英文摘要

We tested the cytotoxic action of 8-hydroxyguanine (8ohG) by observing the viability of several leukemic cell lines (KG-1, U937, Jurkat and K 562) in the presence of 8-hydroxydeoxyguanosine (8ohdG), a nucleoside of 8ohG, It was found that 8ohdG showed cytotoxic action only to KG-1 and that only KG-1 showed a homozygous arginine 209 to glutamine mutation in the hOGG1 gene with an almost negligible hOGG1 enzyme activity, Possibly, the selective cytotoxicity in 8ohdG to KG-1 may be due to its low capacity to cope with an increase in the 8ohG level in DNA resulting from the incorporation of 8ohdG present in the culture media. The mutational impairment of hOGG1 in KG-1 is the first report in leukemic cell lines. Using KG-1 with impaired hOGG1, we demonstrated cytotoxicity of 8ohdG probably due to its incorporation into cellular DNA. This new property of KG-1 may allow it to serve as an useful tool for studies of OGG1, oxidative DNA damage and the cytotoxic action of 8ohG.

语种英语
WOS记录号WOS:000089236800004
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被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58369
专题北京大学药学院
作者单位1.Seoul Natl Univ, Coll Med, Dept Pharmacol, Chongno Gu, Seoul 110744, South Korea
2.Beijing Med Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Korea Canc Ctr Hosp, Lab Radiat Effect, Nowon Gu, Seoul 139706, South Korea
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GB/T 7714
Hyun, JW,Choi, JY,Zeng, HH,et al. Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1[J]. ONCOGENE,2000,19(39):4476-4479.
APA Hyun, JW.,Choi, JY.,Zeng, HH.,Lee, YS.,Kim, HS.,...&Chung, MH.(2000).Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1.ONCOGENE,19(39),4476-4479.
MLA Hyun, JW,et al."Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1".ONCOGENE 19.39(2000):4476-4479.
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