北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1
作者: Hyun, JW; Choi, JY; Zeng, HH; Lee, YS; Kim, HS; Yoon, SH; Chung, MH
关键词: KG-1 ; 8-hydroxyguanine ; hOGG1 ; mutation ; cytotoxicity
刊名: ONCOGENE
发表日期: 2000-09-14
卷: 19, 期:39, 页:4476-4479
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]: REMOVES 8-HYDROXYGUANINE RESIDUES ; OXIDATIVE DNA-DAMAGE ; ESCHERICHIA-COLI ; ENDONUCLEASE ACTIVITY ; OGG1 GENE ; GLYCOSYLASE ; CLONING ; REPAIR ; EXCISION ; PROTEIN
英文摘要:

We tested the cytotoxic action of 8-hydroxyguanine (8ohG) by observing the viability of several leukemic cell lines (KG-1, U937, Jurkat and K 562) in the presence of 8-hydroxydeoxyguanosine (8ohdG), a nucleoside of 8ohG, It was found that 8ohdG showed cytotoxic action only to KG-1 and that only KG-1 showed a homozygous arginine 209 to glutamine mutation in the hOGG1 gene with an almost negligible hOGG1 enzyme activity, Possibly, the selective cytotoxicity in 8ohdG to KG-1 may be due to its low capacity to cope with an increase in the 8ohG level in DNA resulting from the incorporation of 8ohdG present in the culture media. The mutational impairment of hOGG1 in KG-1 is the first report in leukemic cell lines. Using KG-1 with impaired hOGG1, we demonstrated cytotoxicity of 8ohdG probably due to its incorporation into cellular DNA. This new property of KG-1 may allow it to serve as an useful tool for studies of OGG1, oxidative DNA damage and the cytotoxic action of 8ohG.

语种: 英语
WOS记录号: WOS:000089236800004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58369
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Seoul Natl Univ, Coll Med, Dept Pharmacol, Chongno Gu, Seoul 110744, South Korea
2.Beijing Med Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Korea Canc Ctr Hosp, Lab Radiat Effect, Nowon Gu, Seoul 139706, South Korea

Recommended Citation:
Hyun, JW,Choi, JY,Zeng, HH,et al. Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1[J]. ONCOGENE,2000,19(39):4476-4479.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hyun, JW]'s Articles
[Choi, JY]'s Articles
[Zeng, HH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hyun, JW]‘s Articles
[Choi, JY]‘s Articles
[Zeng, HH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace