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学科主题: 基础医学
题名:
Resistin and insulin resistance in hepatocytes: Resistin disturbs glycogen metabolism at the protein level
作者: Yang, Yang; Xiao, Meifang; Mao, Yiqing; Li, Hui; Zhao, Shuyong; Gu, Yi; Wang, Rong; Yu, Jianqiang2; Zhang, Xuemei3; Irwin, David M.4; Niu, Gang5; Tan, Huanran1
关键词: Resistin ; Hepatocyte ; Type 2 diabetes
刊名: BIOMEDICINE & PHARMACOTHERAPY
发表日期: 2009-06-01
DOI: 10.1016/j.biopha.2008.06.033
卷: 63, 期:5, 页:366-374
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: GLUCOSE-UPTAKE ; PHOSPHORYLASE-ACTIVITY ; OBESITY ; MUSCLE ; MICE ; CELLS ; SENSITIVITY ; HEXOKINASE ; EXPRESSION ; SYNTHASE
英文摘要:

Resistin has been considered to link obesity with type 2 diabetes. Liver glycogen metabolism plays an essential role in maintaining glucose homeostasis, we investigated the effect of resistin on liver glycogen metabolism and attempted to identify its role in initiating insulin resistance and type 2 diabetes. Primary culture of rat hepatocytes was treated by resistin and insulin. Glycogen content was determined by the anthrone-reagent method. Real-time PCR, Western blot and enzymatic activity assay were used to detect key enzymes and genes involved in glucose metabolism. Hepatocytes exposed to resistin, but only in the presence of insulin, show a decrease in insulin-stimulated glycogen content. Decreased insulin receptor expression and GS activity and elevated GP activity was observed after the treatment of hepatocytes with resistin. No significant changes in the expression of the genes for these proteins were observed. These results strongly suggest that resistin effects glycogen metabolism at the protein level, and resistin is highly associated with insulin resistance and type 2 diabetes and is a candidate for the prevention and treatment of type 2 diabetes. Our results should lead to the development of novel strategies for the treatment of type 2 diabetes. (C) 2008 Elsevier Masson SAS. All rights reserved.

语种: 英语
所属项目编号: 30772603 ; 2006BAF07B01
项目资助者: National Natural Science Foundation of China (NSFC) ; National Key Scientific and Technology Program
WOS记录号: WOS:000266901900034
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58381
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Ningxia Med Sch, Dept Pharmacol, Ningxia 750004, Peoples R China
3.Dalian Univ Technol, Coll Med, Dept Pharmacol, Dalian 116622, Peoples R China
4.Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5G 1L5, Canada
5.Beijing N&N Genetech Co, Beijing, Peoples R China

Recommended Citation:
Yang, Yang,Xiao, Meifang,Mao, Yiqing,et al. Resistin and insulin resistance in hepatocytes: Resistin disturbs glycogen metabolism at the protein level[J]. BIOMEDICINE & PHARMACOTHERAPY,2009,63(5):366-374.
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