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学科主题: 临床医学
题名:
Brg1-dependent epigenetic control of vascular smooth muscle cell proliferation by hydrogen sulfide
作者: Li, Li1; Liu, Die1; Bu, Dingfang2; Chen, Stella3; Wu, Jianxin4; Tang, Chaoshu5,6; Du, Junbao1,6; Jin, Hongfang1
关键词: Brg1 ; Epigenetic ; Hydrogen sulfide ; Vascular smooth muscle cells ; Proliferation
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
发表日期: 2013-06-01
DOI: 10.1016/j.bbamcr.2013.03.002
卷: 1833, 期:6, 页:1347-1355
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CHROMATIN REMODELING COMPLEXES ; SWI-SNF COMPLEX ; GENE-EXPRESSION ; CARDIOVASCULAR-SYSTEM ; SIGNALING PATHWAYS ; SWI/SNF COMPLEXES ; NITRIC-OXIDE ; FACTOR BRG-1 ; RAT ; GROWTH
英文摘要:

Hydrogen sulfide (H2S) can modulate the proliferation of vascular smooth muscle cells. This study was designed to investigate the epigenetic control of vascular smooth muscle cell proliferation in response to H2S. Microarray analysis indicated that Brahma-related gene 1 (Brg1) and proliferation-related genes including proliferating cell nuclear antigen (Pcna), neurotrophin 3 (Ntf3) and platelet-derived growth factor subunit A (Pdgf alpha) were significantly downregulated by H2S in endothelin-1-stimulated proliferative vascular smooth muscle cells. Brg1 is the central catalytic subunit of the SWI/SNF apparatus (an ATP-dependent chromatin remodeling complex). Overexpression and knockdown of Brg1 confirmed that Brg1 was crucial for H2S-induced inhibition of vascular smooth muscle cell proliferation. A luciferase reporter assay, real-time PCR and Western blotting demonstrated that H2S inhibited Brg1 transcription and expression. A DNase I hypersensitivity assay revealed that H2S reversed endothelin-1-stimulated Pcna, Ntf3 and Pdgf alpha chromatin remodeling and vascular smooth muscle cell proliferation. A chromatin immunoprecipitation assay indicated that H2S inhibited the recruitment of Brg1 to the Pcna, Ntf3 and Pdgf alpha promoters. The results of this study indicate that H2S inhibits vascular smooth muscle cell proliferation via an epigenetic mechanism involving the inhibition of Brg1 transcription and expression, and by reducing the recruitment of Brg1 to the Pcna, Ntf3 and Pdgf alpha promoter regions. (c) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2011CB503904 ; 2012CB517806 ; 81121061 ; 31130030 ; 30901620 ; 81070212 ; 7122184 ; 7121014
项目资助者: Major Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000318390900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58387
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Cent Lab, Beijing 100034, Peoples R China
3.Univ Calif San Diego, Dept Biochem & Cellular Biol, La Jolla, CA 92093 USA
4.Capital Inst Pediat Res, Cent Lab, Beijing 100020, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
6.Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China

Recommended Citation:
Li, Li,Liu, Die,Bu, Dingfang,et al. Brg1-dependent epigenetic control of vascular smooth muscle cell proliferation by hydrogen sulfide[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2013,1833(6):1347-1355.
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