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学科主题: 药学
题名:
Metabolites identification and multi-component pharmacokinetics of ergostane and lanostane triterpenoids in the anticancer mushroom Antrodia cinnamomea
作者: Qiao, Xue1; Wang, Qi1; Ji, Shuai1; Huang, Yun1; Liu, Ke-di1; Zhang, Zheng-xiang2; Bo, Tao2; Tzeng, Yew-min3; Guo, De-an1; Ye, Min1
关键词: Antrodia cinnamomea ; Ergostane ; Lanostane ; Metabolites identification ; Multi-component pharmacokinetics ; Triterpenoids
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2015-07-10
DOI: 10.1016/j.jpba.2015.04.010
卷: 111, 页:266-276
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: IN-VIVO ; MASS-SPECTROMETRY ; FRUITING BODIES ; RAT PLASMA ; CAMPHORATA ; ACIDS ; CHROMATOGRAPHY ; LICORICE ; EXTRACT ; VITRO
英文摘要:

Antrodia cinnamomea is a precious medicinal mushroom popularly used for adjuvant cancer therapy in Taiwan. Its major bioactive constituents are ergostane and lanostane triterpenoids. Although clinical trials for A. cinnamomea have been recently initiated, its metabolism remains unclear. The present study aims to elucidate the metabolism and pharmacokinetics of A. cinnamomea in rats. After oral administration of an ethanol extract; 18 triterpenoids and 8 biotransformed metabolites were detected in rats plasma by UHPLC/qTOE-MS. Four of the metabolites were prepared by semi-synthesis and fully identified by NMR, while the others were tentatively characterized by comparing with the metabolites of single compounds (antcins B, C, H and K). Furthermore, a multi-component pharmacokinetic study of A. cinnamomea was carried out to monitor the plasma concentrations of 14 triterpenoids (ergostanes 1-3, 5-8, 14-16; lanostanes 9, 10, 17, 19) and 2 metabolites (M5, M6) by LC/MS/MS in rats after oral administration of the ethanol extract (1.0 g/kg). The results showed that ergostanes and Delta(7,9(11)) lanostanes, but not Delta(8) lanostanes, could get into circulation. The low-polarity ergostanes (antcins B and C) undertook hydrogenation (C-3 or C-7 carbonyl groups) or hydroxylation to produce polar metabolites. High-polarity ergostanes (antcins H and K) and Delta(7,9(11)) lanostanes were metabolically stable. We also discovered that ergostanes and lanostanes showed remarkably different pharmacokinetic patterns. The ergostanes were generally absorbed and eliminated rapidly, whereas the lanostanes remained in the plasma at a low concentration for a relatively long time. The results indicate that high-polarity ergostanes are the major plasma-exposed components of A. cinnamomea, and may play an important role in its therapeutic effects. (C) 2015 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81222054 ; 81303294 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
WOS记录号: WOS:000355359100036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58389
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Agilent Technol, Beijing 100102, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Chaoyang Univ Technol, Inst Biochem Sci & Technol, Taichung 41349, Taiwan

Recommended Citation:
Qiao, Xue,Wang, Qi,Ji, Shuai,et al. Metabolites identification and multi-component pharmacokinetics of ergostane and lanostane triterpenoids in the anticancer mushroom Antrodia cinnamomea[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,111:266-276.
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