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学科主题: 基础医学
题名:
Peripheral formalin injection induces long-lasting increases in cyclooxygenase 1 expression by microglia in the spinal cord
作者: Zhang, Fei-Yu; Wan, You; Zhang, Zhen-Kang; Light, Alan R.; Fu, Kai-Yuan
关键词: formalin test ; pain ; prostaglandins ; glia ; COX-1 ; COX-2
刊名: JOURNAL OF PAIN
发表日期: 2007-02-01
DOI: 10.1016/j.jpain.2006.06.006
卷: 8, 期:2, 页:110-117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Clinical Neurology ; Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: INFLAMMATORY PAIN HYPERSENSITIVITY ; INDUCED NEUROPEPTIDE RELEASE ; RAT HIND PAW ; PROSTAGLANDIN E-2 ; UP-REGULATION ; TERM HYPERALGESIA ; MESSENGER-RNA ; NERVE INJURY ; AMINO-ACIDS ; ANTIINFLAMMATORY DRUGS
英文摘要:

Activated glia are a source of substances known to enhance pain, including centrally synthesized prostaglandins. We have previously shown that microglia are activated in the spinal cord following peripheral formalin injection. In the present study, we investigated cyclooxygenase (COX-1 and COX-2) expression in the spinal cord using immunohistochemistry and Western blots in the formalin pain model, to further understand how spinal glia modulate pain processing. We show that both COX-1 and COX-2 are constitutively expressed in the spinal cord. Hind paw formalin injection increased COX-1 expression, beginning at 1 day after injection and lasting at least 2 weeks, the duration of experiments. The COX-2 expression changed considerably less, with a significant increase of COX-2 protein level only observed at 2 h after injection. Double labeling studies showed that COX-1 was expressed in microglia and COX-2 was expressed in neurons. These data indicate that both COX-1 and COX-2 are increased in the spinal cord following formalin injection, but the time course and cellular sources are different, suggesting that both COX-1 (longer time points) and COX-2 (very short time points) may be involved in spinal modulation in the formalin pain model. Our study also suggests that spinal microglial activation may play a role in long-term hyperalgesia through the increased expression of COX-1.

Perspective: This article reports that COX-1 expression by microglia is increased in the spinal cord after peripheral formalin injection into the rat hind paw. This result could potentially help clinicians understand how COX-1 may be involved in pain processing and the role microglial activation plays in pain mechanisms. (c) 2007 by the American Pain Society.

语种: 英语
WOS记录号: WOS:000244628500003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58393
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Ctr TMD & Orofaacial Pain, Beijing 100081, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100081, Peoples R China
3.Univ Utah, Dept Anesthesiol, Salt Lake City, UT USA

Recommended Citation:
Zhang, Fei-Yu,Wan, You,Zhang, Zhen-Kang,et al. Peripheral formalin injection induces long-lasting increases in cyclooxygenase 1 expression by microglia in the spinal cord[J]. JOURNAL OF PAIN,2007,8(2):110-117.
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