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IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科  > 期刊论文
学科主题: 临床医学
题名:
Macrophage coculture enhanced invasion of gastric cancer cells via TGF-beta and BMP pathways
作者: Shen, Zhanlong1,2,3; Kauttu, Tuuli2,3; Cao, Jian1; Seppanen, Hanna2,3; Vainionpaa, Sanna2,3; Ye, Yingjiang1; Wang, Shan1; Mustonen, Harri2,3; Puolakkainen, Pauli2,3
关键词: bone morphogenetic proteins (BMPs) ; gastric cancer ; invasion ; transforming growth factor beta (TGF-beta) ; tumor-associated macrophages (TAMs)
刊名: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
发表日期: 2013-04-01
DOI: 10.3109/00365521.2013.772226
卷: 48, 期:4, 页:466-472
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: TUMOR-ASSOCIATED MACROPHAGES ; GROWTH ; EXPRESSION ; PROGRESSION ; METASTASIS ; CARCINOMA ; SUPPRESSION ; PROMOTES ; SURVIVAL ; LINES
英文摘要:

Objective. Transforming growth factor beta (TGF-beta) superfamily plays an important role in regulating gastric cancer progression. As previously demonstrated, tumor-associated macrophages (TAMs) promoted the invasion of gastric cancer cells in Matrigel. However, the role of TGF-beta superfamily signaling between TAMs and gastric cancer remains unclear. Material and methods. Three-dimensional dynamic migration imaging system was used to detect gastric cancer invasion rate cocultured with macrophages in Matrigel before or after TGF-beta receptor 1 or bone morphogenic protein (BMP) receptor 1A and 1B inhibition; real-time RT-PCR was used to quantitatively investigate gene expression (TGF-beta 1, TGF-beta 2, BMP4, and BMP7, ADAM9, MMP9, TIMP3, VEGF-A, and VEGF-C). Results. TGF-beta 1, TGF-beta 2, BMP4, and BMP7 expressions were increased significantly in macrophages grown with cancer cells as compared to macrophages grown alone. The invasion rate and invasion-related genes expressions of both AGS and Hs-746T gastric cancer cell lines were upregulated by macrophages, although the expression profile was different. Invasion rate and invasion-related genes′ expressions of AGS cells cocultured with macrophages were downregulated significantly after TGF-beta R1 and BMPR1 inhibition. Conclusions. Macrophages associated with tumor might promote gastric cancer cells invasion though enhancing TGF-beta/BMPs signal pathway. Inhibiting TGF-beta/BMPs signal between TAMs and gastric cancer cells might provide a new therapeutic method of gastric cancer.

语种: 英语
所属项目编号: RDB 2012-18
项目资助者: Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds ; Peking University People&prime ; s Hospital Funds
WOS记录号: WOS:000316699600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58398
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Univ Helsinki, FIN-00290 Helsinki, Finland
2.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
3.Univ Helsinki, Cent Hosp, Dept Surg, FIN-00290 Helsinki, Finland

Recommended Citation:
Shen, Zhanlong,Kauttu, Tuuli,Cao, Jian,et al. Macrophage coculture enhanced invasion of gastric cancer cells via TGF-beta and BMP pathways[J]. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY,2013,48(4):466-472.
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