|Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo|
|Zheng, Xi-Yuan1,2; Yang, Ying-Fan1,2; Li, Wan1,2; Zhao, Xin1; Sun, Yi1; Sun, Hua3,4; Wang, Yue-Hua3,4; Pu, Xiao-Ping1|
|关键词||Xanthones Swertia punicea Hemsl Hepatoprotective CCl4-induced hepatotoxicity Acetaminophen-induced hepatotoxicity DMN-induced rat hepatic fibrosis|
|刊名||JOURNAL OF ETHNOPHARMACOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy|
|研究领域[WOS]||Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine|
|关键词[WOS]||INDUCED OXIDATIVE DAMAGE ; INDUCED LIVER FIBROSIS ; HEPG2 CELLS ; COVALENT BINDING ; INJURY ; RATS ; HEPATOTOXICITY ; STRESS ; CYTOTOXICITY ; PROTEINS|
Ethnopharmacological relevance: Swertia punicea Hemsl. (Gentianaceae) is more commonly known as "Ganyan-cao" and used mainly as a traditional Chinese folk medicine for the treatment of acute bilious hepatitis, cholecystitis, fever, intoxification and jaundice.
Materials and methods: The active hepatoprotective constituents of Swertia punicea were purified using various column chromatography techniques. The structures of two isolated compounds were determined on the basis of spectroscopic data interpretation such as NMR analysis. The hepatoprotective activities of isolated compounds were evaluated by using hepatotoxicity in vitro and dimethylnitrosamine-induced rat hepatic fibrosis in vivo, respectively.
Results: Two xanthones, 1, 7-dihydroxy-3, 4, 8-trimethoxyxanthone (1) and bellidifolin (2) were isolated from the stems of Swertia punicea. The compounds 1 and 2 exhibited notable hepatoprotective activities against carbon tetrachloride (CCl4) -induced HepG2 cell damage, and effectively alleviated the levels of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malonic dialdehyde (MDA) induced by CCl4 in a concentration-dependent manner. Co-treatment with compound 2 significantly increased the cell viability compared with N-acetyl-p-aminophenol (APAP) treatment. Compound 2 also alleviated APAP-induced hepatotoxicity by increasing glutathione (GSH) content and decreasing hydroxyl free radical (.OH) levels and reactive oxygen specises (ROS) production. In addition, the protective effect of compound 1 significantly alleviated DMN-induced liver inflammation and fibrosis. Oral administration of compound 1 recovered the reduction of albumin (ALB) and reversed the elevation of serum alanine transaminase (ALT), AST and total bilirubin (TBIL) in dimethylnitrosamine (DMN)-induced fibrotic rats. Severe oxidative stress induced in fibrotic rats was evidenced by a 1.5-fold elevation in MDA and a fall in the SOD activity, and treatment with compound 1 protected against these adverse effects. Recovery of rat liver tissue against DMN-induced hepatocellular necrosis, inflammatory changes and hepatic fibrosis by compound 1 is also confirmed by H&E and Masson stained histopathological evaluation of liver tissue.
Conclusion: Two xanthones from Swertia punicea exhibited hepatoprotective activities in vitro (compounds 1 and 2) and in vivo (compound 1), respectively. (c) 2014 Elsevier Ireland Ltd. All rights reserved.
|项目编号||81202937 ; Founder 201004 ; 2012ZX09103201-042|
|资助机构||National Natural Science Foundation of China ; Innovative Drug Research Fund of Founder Group & ; PekingUniversity Health Science Center ; Significant New Drugs Creation "Five-Year Plan" Special Science and Technology Major|
|作者单位||1.Peking Univ, Natl Key Res Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China|
2.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
3.Chinese Acad Med Sci, Inst Mat Med, Beijing, Peoples R China
4.Peking Union Med Coll, Beijing, Peoples R China
|Zheng, Xi-Yuan,Yang, Ying-Fan,Li, Wan,et al. Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,153(3):854-863.|
|APA||Zheng, Xi-Yuan.,Yang, Ying-Fan.,Li, Wan.,Zhao, Xin.,Sun, Yi.,...&Pu, Xiao-Ping.(2014).Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo.JOURNAL OF ETHNOPHARMACOLOGY,153(3),854-863.|
|MLA||Zheng, Xi-Yuan,et al."Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo".JOURNAL OF ETHNOPHARMACOLOGY 153.3(2014):854-863.|