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Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo
Zheng, Xi-Yuan1,2; Yang, Ying-Fan1,2; Li, Wan1,2; Zhao, Xin1; Sun, Yi1; Sun, Hua3,4; Wang, Yue-Hua3,4; Pu, Xiao-Ping1
关键词Xanthones Swertia punicea Hemsl Hepatoprotective CCl4-induced hepatotoxicity Acetaminophen-induced hepatotoxicity DMN-induced rat hepatic fibrosis
刊名JOURNAL OF ETHNOPHARMACOLOGY
2014-05-14
DOI10.1016/j.jep.2014.03.058
153期:3页:854-863
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]INDUCED OXIDATIVE DAMAGE ; INDUCED LIVER FIBROSIS ; HEPG2 CELLS ; COVALENT BINDING ; INJURY ; RATS ; HEPATOTOXICITY ; STRESS ; CYTOTOXICITY ; PROTEINS
英文摘要

Ethnopharmacological relevance: Swertia punicea Hemsl. (Gentianaceae) is more commonly known as "Ganyan-cao" and used mainly as a traditional Chinese folk medicine for the treatment of acute bilious hepatitis, cholecystitis, fever, intoxification and jaundice.

Materials and methods: The active hepatoprotective constituents of Swertia punicea were purified using various column chromatography techniques. The structures of two isolated compounds were determined on the basis of spectroscopic data interpretation such as NMR analysis. The hepatoprotective activities of isolated compounds were evaluated by using hepatotoxicity in vitro and dimethylnitrosamine-induced rat hepatic fibrosis in vivo, respectively.

Results: Two xanthones, 1, 7-dihydroxy-3, 4, 8-trimethoxyxanthone (1) and bellidifolin (2) were isolated from the stems of Swertia punicea. The compounds 1 and 2 exhibited notable hepatoprotective activities against carbon tetrachloride (CCl4) -induced HepG2 cell damage, and effectively alleviated the levels of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malonic dialdehyde (MDA) induced by CCl4 in a concentration-dependent manner. Co-treatment with compound 2 significantly increased the cell viability compared with N-acetyl-p-aminophenol (APAP) treatment. Compound 2 also alleviated APAP-induced hepatotoxicity by increasing glutathione (GSH) content and decreasing hydroxyl free radical (.OH) levels and reactive oxygen specises (ROS) production. In addition, the protective effect of compound 1 significantly alleviated DMN-induced liver inflammation and fibrosis. Oral administration of compound 1 recovered the reduction of albumin (ALB) and reversed the elevation of serum alanine transaminase (ALT), AST and total bilirubin (TBIL) in dimethylnitrosamine (DMN)-induced fibrotic rats. Severe oxidative stress induced in fibrotic rats was evidenced by a 1.5-fold elevation in MDA and a fall in the SOD activity, and treatment with compound 1 protected against these adverse effects. Recovery of rat liver tissue against DMN-induced hepatocellular necrosis, inflammatory changes and hepatic fibrosis by compound 1 is also confirmed by H&E and Masson stained histopathological evaluation of liver tissue.

Conclusion: Two xanthones from Swertia punicea exhibited hepatoprotective activities in vitro (compounds 1 and 2) and in vivo (compound 1), respectively. (c) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000337648800035
项目编号81202937 ; Founder 201004 ; 2012ZX09103201-042
资助机构National Natural Science Foundation of China ; Innovative Drug Research Fund of Founder Group &amp ; PekingUniversity Health Science Center ; Significant New Drugs Creation "Five-Year Plan" Special Science and Technology Major
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58405
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Natl Key Res Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
3.Chinese Acad Med Sci, Inst Mat Med, Beijing, Peoples R China
4.Peking Union Med Coll, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Xi-Yuan,Yang, Ying-Fan,Li, Wan,et al. Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,153(3):854-863.
APA Zheng, Xi-Yuan.,Yang, Ying-Fan.,Li, Wan.,Zhao, Xin.,Sun, Yi.,...&Pu, Xiao-Ping.(2014).Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo.JOURNAL OF ETHNOPHARMACOLOGY,153(3),854-863.
MLA Zheng, Xi-Yuan,et al."Two xanthones from Swertia punicea with hepatoprotective activities in vitro and in vivo".JOURNAL OF ETHNOPHARMACOLOGY 153.3(2014):854-863.
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