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学科主题: 临床医学
题名:
Ultrasound Molecular Imaging of Acute Cellular Cardiac Allograft Rejection in Rat With T-Cell-Specific Nanobubbles
作者: Wu, Wei1; Zhang, Zhe2; Zhuo, Lisha3; Zhou, Lina4; Liu, Ping3; He, Yun4; Gao, Yunhua3; Li, Rui4; Chen, Qinghai4; Hua, Xing1,4
关键词: Myocardial contrast echocardiography ; Molecular imaging ; Nanobubbles ; Acute cellular rejection ; Cardiac transplant
刊名: TRANSPLANTATION
发表日期: 2013-09-01
DOI: 10.1097/TP.0b013e31829b759f
卷: 96, 期:6, 页:543-549
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Surgery ; Transplantation
研究领域[WOS]: Immunology ; Surgery ; Transplantation
关键词[WOS]: HEART-TRANSPLANTATION ; ENHANCED ULTRASOUND ; THERAPY
英文摘要:

Background. Acute rejection (AR) is one of the main obstacles of cardiac transplantation; however, a noninvasive diagnostic method, which reflects its pathologic nature, has not been developed yet. In this study, we prepared a specific nanobubbles targeting to the activated T cells and applied it in the ultrasound molecular imaging of AR in heart transplantation by myocardial contrast echocardiography (MCE).

Methods. Nanobubbles loading anti-CD25 antibody (NBspecific) or isotype control antibody (NBnonspecific) were prepared and then applied in the ultrasound molecular imaging by MCE in a rat model. MCE was performed in 24 allografts and 18 isografts that were divided into three groups, including days 2, 4, and 6 after transplantation. Confocal laser scanning microscopy was used to evaluate the binding of nanobubbles and T cells in four allografts and four isografts.

Results. MCE with NBspecific in allograft showed a "delayed enhancement," and the time-intensity curve presented a second peak. The intensity and time of second peak were both positively correlated with the transplant time (P<0.01) and the pathologic grade of AR (P<0.01). Confocal laser scanning microscopy demonstrated the binding of nanobubbles and lymphocytes in myocardium post-MCE with NBspecific.

Conclusions. Ultrasound molecular imaging of AR after heart transplantation can be achieved by using MCE with the nanobubbles targeted to T cells. The appearance of delayed enhancement indicates the occurrence of AR, and the intensity and time of the second peak in time-intensity curve provide potential quantitative indications for diagnosis and severity of AR.

语种: 英语
所属项目编号: 30801060 ; 81000142
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000330383900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58408
Appears in Collections:北京大学第三临床医学院_心脏外科_期刊论文

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作者单位: 1.Third Mil Univ, Southwest Hosp, Dept Cardiothorac Surg, Chongqing 400038, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiac Surg, Beijing 100871, Peoples R China
3.Third Mil Univ, Xinqiao Hosp, Dept Ultrasound, Chongqing 400038, Peoples R China
4.Third Mil Univ, Southwest Hosp, Dept Ultrasound, Chongqing 400038, Peoples R China

Recommended Citation:
Wu, Wei,Zhang, Zhe,Zhuo, Lisha,et al. Ultrasound Molecular Imaging of Acute Cellular Cardiac Allograft Rejection in Rat With T-Cell-Specific Nanobubbles[J]. TRANSPLANTATION,2013,96(6):543-549.
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