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学科主题基础医学
PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers
Zhong, Jian; Zheng, Wenfu; Huang, Lixin; Hong, Yuankai; Wang, Lijun; Qiu, Yang; Sha, Yinlin
关键词in situ time-lapse AFM supported lipid bilayers (SLBs) PrP106-126 amide semi-penetrated potation flat high-rise domains (FHDs)
刊名BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
2007-06-01
DOI10.1016/j.bbamen.2007.03.003
1768期:6页:1420-1429
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; PRION PROTEIN-FRAGMENT ; ANTIMICROBIAL PEPTIDES ; MEMBRANE INTERACTIONS ; ALZHEIMERS-DISEASE ; MODEL MEMBRANES ; ALKALINE-PHOSPHATASE ; RAFT FORMATION ; CHOLESTEROL ; NEUROTOXICITY
英文摘要

A major hallmark of prion diseases is the cerebral amyloid accumulation of the pathogenic PrPSc, an abnormally misfolded, protease-resistant, and beta-sheet rich protein. PrP106-126 is the key domain responsible for the conformational conversion and aggregation of PrP. It shares important physicochemical characteristics with PrPSc and presents similar neurotoxicity as PrPSc. By combination of fluorescence polarization, dye release assay and in situ time-lapse atomic force microscopy (AFM), we investigated the PrP106-126 amide interacting with the large unilamellar vesicles (LUVs) and the supported lipid bilayers (SLBs). The results suggest that the interactions involve a poration-mediated process: firstly, the peptide binding results in the formation of pores in the membranes, which penetrate only half of the membranes; subsequently, PrP106-126 amide undergoes the poration-mediated diffusion in the SLBs, represented by the formation and expansion of the flat high-rise domains (FHDs). The possible mechanisms of the interactions between PrP106-126 amide and lipid membranes are proposed based on our observations. (c) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000247455700009
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58412
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Biomed X Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Molecule & Nanobiol Lab, Beijing 100083, Peoples R China
3.Univ Chicago, Dept Biochem & Mol Biophys, Chicago, IL 60637 USA
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Zhong, Jian,Zheng, Wenfu,Huang, Lixin,et al. PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2007,1768(6):1420-1429.
APA Zhong, Jian.,Zheng, Wenfu.,Huang, Lixin.,Hong, Yuankai.,Wang, Lijun.,...&Sha, Yinlin.(2007).PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1768(6),1420-1429.
MLA Zhong, Jian,et al."PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1768.6(2007):1420-1429.
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