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学科主题: 基础医学
题名:
PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers
作者: Zhong, Jian; Zheng, Wenfu; Huang, Lixin; Hong, Yuankai; Wang, Lijun; Qiu, Yang; Sha, Yinlin
关键词: in situ time-lapse AFM ; supported lipid bilayers (SLBs) ; PrP106-126 amide ; semi-penetrated potation ; flat high-rise domains (FHDs)
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
发表日期: 2007-06-01
DOI: 10.1016/j.bbamen.2007.03.003
卷: 1768, 期:6, 页:1420-1429
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; PRION PROTEIN-FRAGMENT ; ANTIMICROBIAL PEPTIDES ; MEMBRANE INTERACTIONS ; ALZHEIMERS-DISEASE ; MODEL MEMBRANES ; ALKALINE-PHOSPHATASE ; RAFT FORMATION ; CHOLESTEROL ; NEUROTOXICITY
英文摘要:

A major hallmark of prion diseases is the cerebral amyloid accumulation of the pathogenic PrPSc, an abnormally misfolded, protease-resistant, and beta-sheet rich protein. PrP106-126 is the key domain responsible for the conformational conversion and aggregation of PrP. It shares important physicochemical characteristics with PrPSc and presents similar neurotoxicity as PrPSc. By combination of fluorescence polarization, dye release assay and in situ time-lapse atomic force microscopy (AFM), we investigated the PrP106-126 amide interacting with the large unilamellar vesicles (LUVs) and the supported lipid bilayers (SLBs). The results suggest that the interactions involve a poration-mediated process: firstly, the peptide binding results in the formation of pores in the membranes, which penetrate only half of the membranes; subsequently, PrP106-126 amide undergoes the poration-mediated diffusion in the SLBs, represented by the formation and expansion of the flat high-rise domains (FHDs). The possible mechanisms of the interactions between PrP106-126 amide and lipid membranes are proposed based on our observations. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000247455700009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58412
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Biomed X Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Molecule & Nanobiol Lab, Beijing 100083, Peoples R China
3.Univ Chicago, Dept Biochem & Mol Biophys, Chicago, IL 60637 USA

Recommended Citation:
Zhong, Jian,Zheng, Wenfu,Huang, Lixin,et al. PrP106-126 amide causes the semi-penetrated poration in the supported lipid bilayers[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2007,1768(6):1420-1429.
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