|Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics|
|Jia, Shu-Qin1,4; Niu, Zhao-Jian2; Zhang, Lian-Hai1; Zhong, Xi-Yao1; Shi, Tao1; Du, Hong3; Zhang, Gui-Guo3; Hu, Ying3; Su, Xiu-Lan4; Ji, Jia-Fu1|
|关键词||Stomach neoplasms Mass spectrometry Proteomics Prognosis S100P|
|刊名||JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||GENE-EXPRESSION ; PANCREATIC-CANCER ; BINDING PROTEIN ; POOR-PROGNOSIS ; MS/MS ANALYSIS ; S100 GENES ; CARCINOMA ; SURVIVAL ; CELLS ; ADENOCARCINOMA|
The objective of this study was to identify differentially expressed proteins of advanced gastric cancer from patients with different prognosis using NanoLC-MS/MS (LTQ) (nanoflow liquid chromatography system interfaced with a linear ion trap LTQ mass spectrometer).
Eight gastric cancer patients with relatively early TNM stage and survival time > 34 months were identified as good survival (group G), while the other eight with late stage and survival time < 15 months as poor survival (group P). The total protein of the tissue samples from each group was extracted and pooled together respectively. The resulting two protein mixtures were trypsin-digested and analyzed using NanoLC-MS/MS (LTQ). Database searches were done against NCBI non-redundant database and SWISS-PROT database and the identified proteins were classified through an online Web Gene Ontology Annotation Plot tool. Immunohistochemistry was used to verify candidate prognosis-related proteins.
There were 284 and 213 proteins identified for group G and group P respectively. And 117 proteins were detected exclusively in group G and 46 proteins exclusively in group P. These protein markers function in calcium ion signaling pathway, cellular metabolism, cytoskeleton formation, stress reaction, etc. Among those, the down-regulated expression of S100P was verified to claim a poor clinical outcome of gastric cancer patients (P = 0.0375).
The MS-based proteomics approach is efficient in identifying differentially expressed proteins in relation to prognosis of advanced gastric cancer patients. These differentially expressed proteins could be potential prognosis-related cancer markers and deserve further validation and functional study.
|项目编号||30571807 ; D0905001040631|
|资助机构||National Natural Science Foundation of China ; Foundation of Beijing Municipal Committee of Science & ; Technology, China|
|作者单位||1.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Surg,Beijing Inst Canc Res, Beijing 100036, Peoples R China|
2.Shandong Univ, Qilu Hosp, Dept Gen Surg, Jinan 250012, Shandong, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp, Tissue Bank,Beijing Inst Canc Res, Beijing 100036, Peoples R China
4.Inner Mongolia Med Coll, Clin Med Res Ctr, Hohhot 010050, Peoples R China
|Jia, Shu-Qin,Niu, Zhao-Jian,Zhang, Lian-Hai,et al. Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2009,135(3):403-411.|
|APA||Jia, Shu-Qin.,Niu, Zhao-Jian.,Zhang, Lian-Hai.,Zhong, Xi-Yao.,Shi, Tao.,...&Ji, Jia-Fu.(2009).Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,135(3),403-411.|
|MLA||Jia, Shu-Qin,et al."Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 135.3(2009):403-411.|