IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics
Jia, Shu-Qin1,4; Niu, Zhao-Jian2; Zhang, Lian-Hai1; Zhong, Xi-Yao1; Shi, Tao1; Du, Hong3; Zhang, Gui-Guo3; Hu, Ying3; Su, Xiu-Lan4; Ji, Jia-Fu1
关键词Stomach neoplasms Mass spectrometry Proteomics Prognosis S100P
刊名JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
2009-03-01
DOI10.1007/s00432-008-0474-3
135期:3页:403-411
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]GENE-EXPRESSION ; PANCREATIC-CANCER ; BINDING PROTEIN ; POOR-PROGNOSIS ; MS/MS ANALYSIS ; S100 GENES ; CARCINOMA ; SURVIVAL ; CELLS ; ADENOCARCINOMA
英文摘要

The objective of this study was to identify differentially expressed proteins of advanced gastric cancer from patients with different prognosis using NanoLC-MS/MS (LTQ) (nanoflow liquid chromatography system interfaced with a linear ion trap LTQ mass spectrometer).

Eight gastric cancer patients with relatively early TNM stage and survival time > 34 months were identified as good survival (group G), while the other eight with late stage and survival time < 15 months as poor survival (group P). The total protein of the tissue samples from each group was extracted and pooled together respectively. The resulting two protein mixtures were trypsin-digested and analyzed using NanoLC-MS/MS (LTQ). Database searches were done against NCBI non-redundant database and SWISS-PROT database and the identified proteins were classified through an online Web Gene Ontology Annotation Plot tool. Immunohistochemistry was used to verify candidate prognosis-related proteins.

There were 284 and 213 proteins identified for group G and group P respectively. And 117 proteins were detected exclusively in group G and 46 proteins exclusively in group P. These protein markers function in calcium ion signaling pathway, cellular metabolism, cytoskeleton formation, stress reaction, etc. Among those, the down-regulated expression of S100P was verified to claim a poor clinical outcome of gastric cancer patients (P = 0.0375).

The MS-based proteomics approach is efficient in identifying differentially expressed proteins in relation to prognosis of advanced gastric cancer patients. These differentially expressed proteins could be potential prognosis-related cancer markers and deserve further validation and functional study.

语种英语
WOS记录号WOS:000263022300010
项目编号30571807 ; D0905001040631
资助机构National Natural Science Foundation of China ; Foundation of Beijing Municipal Committee of Science &amp ; Technology, China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58413
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_胃肠肿瘤中心一病区
北京大学临床肿瘤学院_标本库
作者单位1.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Surg,Beijing Inst Canc Res, Beijing 100036, Peoples R China
2.Shandong Univ, Qilu Hosp, Dept Gen Surg, Jinan 250012, Shandong, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp, Tissue Bank,Beijing Inst Canc Res, Beijing 100036, Peoples R China
4.Inner Mongolia Med Coll, Clin Med Res Ctr, Hohhot 010050, Peoples R China
推荐引用方式
GB/T 7714
Jia, Shu-Qin,Niu, Zhao-Jian,Zhang, Lian-Hai,et al. Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2009,135(3):403-411.
APA Jia, Shu-Qin.,Niu, Zhao-Jian.,Zhang, Lian-Hai.,Zhong, Xi-Yao.,Shi, Tao.,...&Ji, Jia-Fu.(2009).Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,135(3),403-411.
MLA Jia, Shu-Qin,et al."Identification of prognosis-related proteins in advanced gastric cancer by mass spectrometry-based comparative proteomics".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 135.3(2009):403-411.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jia, Shu-Qin]的文章
[Niu, Zhao-Jian]的文章
[Zhang, Lian-Hai]的文章
百度学术
百度学术中相似的文章
[Jia, Shu-Qin]的文章
[Niu, Zhao-Jian]的文章
[Zhang, Lian-Hai]的文章
必应学术
必应学术中相似的文章
[Jia, Shu-Qin]的文章
[Niu, Zhao-Jian]的文章
[Zhang, Lian-Hai]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。