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学科主题: 临床医学
题名:
Sandwich ELISA for detecting urinary Survivin in bladder cancer
作者: Li, Xuefeng; Wang, Yaming; Xu, Jianjun; Zhang, Qingyun
关键词: Survivin ; bladder cancer ; enzyme-linked immunosorbent assay (ELISA) ; tumor marker ; diagnosis
刊名: CHINESE JOURNAL OF CANCER RESEARCH
发表日期: 2013-08-01
DOI: 10.3978/j.issn.1000-9604.2013.08.11
卷: 25, 期:4, 页:375-381
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CELL-DIVISION ; LUNG-CANCER ; EXPRESSION ; APOPTOSIS ; DIAGNOSIS ; PROGNOSIS ; BIOMARKER ; MARKER
英文摘要:

Objective: Survivin as a tumor marker in the diagnosis of bladder cancer has not been completely confirmed yet and there are few reports about using Survivin enzyme-linked immunosorbent assay (ELISA) kit to detect the urine of bladder cancer patients. This study aimed to develop a Survivin ELISA and validate its value in the detection of bladder cancer.

Methods: Through square matrix titration, different combinations of coating antibody and detecting antibody, a Survivin ELISA was constructed. This assay was evaluated according to intra-assay precision, inter-assay precision and minimum detectable dose (MDD). Survivin levels were detected and analyzed in 102 bladder cancer patients and 102 healthy people by established ELISA. Then cutoff value was defined according to the analysis of receiver operating characteristic (ROC) curve. The sensitivity and specificity of detection were calculated on the basis of cutoff value to diagnose bladder cancer patients. Furthermore, the value of Survivin expression detected by ELISA among different clinicopathological characteristics of patients was also compared.

Results: Through optimization of different conditions, intra-assay precision was 8.39%, inter-assay precision 8.57% and MDD 0.0625 ng/mL in this assay. When the optical density at 450 nm (OD450) was 0.09, it could get the optimized diagnostic cutoff value. According to this value, the sensitivity and specificity of diagnosis in bladder cancer patients were 70.6% and 89.2%, respectively. The associations between patients′ clinical variables and OD450 were not significant except tumor numbers in patients.

Conclusions: This experiment has preliminarily developed a Survivin ELISA and confirmed Survivin as a biomarker which owned a practical and significant value in the diagnosis of bladder cancer.

语种: 英语
所属项目编号: 2012AA02A504 ; 2011-1009-03 ; Z121107005112004
项目资助者: National High Technology Research Development Plan ; Capital Healthy Development Special Fund ; Capital Laboratory Medicine Clinical Characteristic Fund
WOS记录号: WOS:000324003700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58424
Appears in Collections:北京大学临床肿瘤学院_临床实验室_期刊论文

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作者单位: Peking Univ, Canc Hosp & Inst, Dept Clin Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China

Recommended Citation:
Li, Xuefeng,Wang, Yaming,Xu, Jianjun,et al. Sandwich ELISA for detecting urinary Survivin in bladder cancer[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25(4):375-381.
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