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Implications for Differentiation of Endogenous Stem Cells: Therapeutic Effect from Icariside II on a Rat Model of Postprostatectomy Erectile Dysfunction
Xu, Yongde1; Guan, Ruili1; Lei, Hongen1; Gao, Zhezhu1; Li, Huixi2; Hui, Yu3; Zhou, Feng3; Wang, Lin2; Lin, Guiting2; Xin, Zhongcheng1
刊名STEM CELLS AND DEVELOPMENT
2015-03-15
DOI10.1089/scd.2014.0380
24期:6页:747-755
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
资助者National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]LABEL-RETAINING CELLS ; ACTIVATED PROTEIN-KINASE ; CAVERNOUS NERVE INJURY ; ICARIIN ; IDENTIFICATION ; PROSTATECTOMY ; RECOVERY ; PATHWAY ; TISSUE ; MAPK
英文摘要

Self-renewal and differentiation of endogenous stem cells (SCs) are essential for adult tissue homoeostasis and intrinsic healing capacity. In this study, we hypothesize that penis contains a small population of endogenous SCs, which might help rejuvenation of damaged erectile function. In this study, 60 newborn male rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU; 50 mg/kg) for the purpose of tracking endogenous SCs. Twelve weeks later, 48 rats underwent bilateral cavernous nerves injury and were randomized into gavage feeding of solvent (vehicle group) or icariside II (0.5, 1.5, and 4.5 mg/kg/day, respectively). Twelve sham-operated rats received vehicle treatment and served as control. The treatments were continued for 4 weeks followed by a washout period of 72 h. Results showed that ICA II treatment significantly restored erectile function and effectively prevented distortion of normal neural anatomy, smooth muscle atrophy, and collagen deposition compared with the vehicle group. The numbers of label-retaining cells (LRCs) coexpressing EdU and differentiated phenotypes (smooth muscle marker alpha-SMA or Schwann cell marker S100) were significantly higher in the three ICA II-treated groups than those in vehicle group in a dose-dependent manner. In addition, the changing trend of p38 mitogen-activated protein kinase (MAPK) activity in the penis between groups was same as that of the number of differentiated LRCs. Together, these results suggest that the underlying mechanisms of ICA II in ameliorating erectile function and pathological changes appear to involve enhanced endogenous SCs differentiation, which might be regulated by p38 MAPK signaling pathway.

语种英语
所属项目编号81272531 ; 81300478 ; BK20130269
资助者National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province
WOS记录号WOS:000350459100007
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58438
专题北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Mol Biol Lab, Beijing 100034, Peoples R China
2.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
3.Soochow Univ, Affiliated Hosp 1, Dept Urol, Suzhou, Peoples R China
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GB/T 7714
Xu, Yongde,Guan, Ruili,Lei, Hongen,et al. Implications for Differentiation of Endogenous Stem Cells: Therapeutic Effect from Icariside II on a Rat Model of Postprostatectomy Erectile Dysfunction[J]. STEM CELLS AND DEVELOPMENT,2015,24(6):747-755.
APA Xu, Yongde.,Guan, Ruili.,Lei, Hongen.,Gao, Zhezhu.,Li, Huixi.,...&Xin, Zhongcheng.(2015).Implications for Differentiation of Endogenous Stem Cells: Therapeutic Effect from Icariside II on a Rat Model of Postprostatectomy Erectile Dysfunction.STEM CELLS AND DEVELOPMENT,24(6),747-755.
MLA Xu, Yongde,et al."Implications for Differentiation of Endogenous Stem Cells: Therapeutic Effect from Icariside II on a Rat Model of Postprostatectomy Erectile Dysfunction".STEM CELLS AND DEVELOPMENT 24.6(2015):747-755.
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