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IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室  > 期刊论文
学科主题: 临床医学
题名:
Downregulation of 425G > A variant of calcium-binding protein S100A14 associated with poor differentiation and prognosis in gastric cancer
作者: Zhang, Qingying1; Zhu, Min1; Cheng, Wenxiang1; Xing, Rui2; Li, Wenmei2; Zhao, Min2; Xu, Liyan3; Li, Enmin3; Luo, Guangbin4; Lu, Youyong2
关键词: S100A14 ; Gastric cancer ; Differentiation ; Genetic variant ; Prognosis
刊名: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
发表日期: 2015-04-01
DOI: 10.1007/s00432-014-1830-0
卷: 141, 期:4, 页:691-703
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: BREAST-CANCER ; EXPRESSION ; CARCINOMA ; CELLS ; METASTASIS ; ROLES ; FAMILY ; OVEREXPRESSION ; MODULATOR ; PROMOTES
英文摘要:

Altered level of S100 calcium-binding proteins is involved in tumor development and progression. However, their role in gastric cancer (GC) is not well documented. We investigated the expression pattern of S100 proteins and differentiation or prognosis as well as possible mechanisms in GC.

RT-PCR, Western blot analysis, and immunohistochemistry were used to determine the mRNA and protein expression of S100 family genes in GC. The polymorphisms of promoter and 5′-UTR of S100A14 gene were identified and related to luciferase reporter gene activity. Association of S100A14 expression with clinicopathologic features and survival in GC was analyzed.

We detected upregulated S100A2, S100A6, S100A10, and S100A11 expression and downregulated S100P and S100B expression in GC. Particularly, we detected differential mRNA and protein expression of S100A14 in GC cell lines and primary tumors. Furthermore, S100A14 expression change was related to a differentiated GC phenotype, with an expression in 31/40 (77.5 %) samples of well-differentiated tumors and 29/85 (34.1 %) samples of poorly differentiated tumors (P < 0.001). Moreover, 5-year survival was better in GC cases with positive than negative S100A14 level (P = 0.02). The genetic variant 425G > A on the 5′-UTR of S100A14 was associated with reduced S100A14 expression in GC cells.

Decreased expression of S100A14 with presence of its genetic variant 425G > A may be associated with an undifferentiated phenotype and poor prognosis in GC.

语种: 英语
所属项目编号: 2004 CB518708 ; 2001AA233061 ; 2002-BA711A11 ; 2012AA02A203
项目资助者: State Key Basic Research Program ; National Bio-Tech 86-3 program
WOS记录号: WOS:000351206900010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58441
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: 1.Shantou Univ Med Coll, Dept Prevent Med, Shantou 515041, Guangdong, Peoples R China
2.Peking Univ Canc Hosp Inst, Minist Educ, Key Lab Carcinogenesis & Translat Res, Mol Oncol Lab, Beijing 100142, Peoples R China
3.Shantou Univ Med Coll, Dept Chem, Shantou 515041, Guangdong, Peoples R China
4.Case Western Reserve Univ, Dept Genet Ireland Canc Ctr, Cleveland, OH 44106 USA

Recommended Citation:
Zhang, Qingying,Zhu, Min,Cheng, Wenxiang,et al. Downregulation of 425G &gt; A variant of calcium-binding protein S100A14 associated with poor differentiation and prognosis in gastric cancer[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2015,141(4):691-703.
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