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Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells
Zhao, Peng1; Zhong, Weijian2; Ying, Xianping2; Yao, Biyun1; Yuan, Zhun1; Fu, Juanling1; Zhou, Zongcan1
关键词Anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) Anti-BPDE-transformed cells Two-dimensional gel electrophoresis Q-TOF MS/MS PCNA TPD52
刊名CHEMICO-BIOLOGICAL INTERACTIONS
2010-07-30
DOI10.1016/j.cbi.2010.04.015
186期:2页:166-173
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]POLYCYCLIC AROMATIC-HYDROCARBONS ; HUMAN PROSTATE-CANCER ; TUMOR PROTEIN D52 ; GENE-EXPRESSION ; NUCLEAR ANTIGEN ; LUNG-CANCER ; PCNA ; CARCINOMA ; APOPTOSIS ; CYCLE
英文摘要

In the present study, we investigated the proteomic profiling of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (anti-BPDE)-transformed human bronchial epithelial cell line (16HBE-C) and its parental cell line (16HBE) G0/G1 cells. Differential analysis of proteomic profiling indicated that 67 polypeptides were down-regulated and 77 polypeptides were up-regulated in 16HBE-CG0/G1 cells compared to 16HBE G0/G1 cells. Then 16 differentially expressed protein spots were analyzed with Q-TOF MS/MS. Of these spots, 3 down-regulated polypeptides were identified as sorcin, small ubiquitin-related modifier 2 precursor and eukaryotic translation initiation factor 5A-1, and 9 up-regulated polypeptides were identified as calmodulin, myosin light polypeptide 6, eukaryotic translation initiation factor 6. proliferating cell nuclear antigen (PCNA), tumor protein D52 (TPD52), superoxide dismutase [Cu-Zn], prohibitin, nuclear protein Hcc-1 and vimentin. These proteins are involved in cell proliferation, protein synthesis, signal transduction and carcinogenesis. Western blotting analysis verified the increased expression levels of PCNA and TPD52 in 16HBE-C G0/G1 cells. Based on the clues from proteomic analysis, the migration and invasion capabilities of 16HBE-C and 16HBE cells were tested. The results indicated that 16HBE-C cells showed much higher migration and invasion capabilities than 16HBE cells, and moreover, the suppression of TPD52 by RNAi resulted in significant decrease of migration and invasion capabilities of 16HBE-C cells. These results will be valuable for further investigating and understanding the mechanisms underlying BaP-induced carcinogenesis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000279644900009
项目编号30471470 ; 30671781 ; 2002CB512906 ; BMU20090460
资助机构National Nature Science Foundation of China ; China National Key Basic Research and Development Program ; Science Foundation of Peking University Health Science Center
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58451
专题北京大学公共卫生学院_毒理学系
北京大学公共卫生学院_公共卫生学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China
2.Shanghai Municipal Ctr Dis Control & Prevent, Dept Toxicol, Shanghai 200336, Peoples R China
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GB/T 7714
Zhao, Peng,Zhong, Weijian,Ying, Xianping,et al. Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2010,186(2):166-173.
APA Zhao, Peng.,Zhong, Weijian.,Ying, Xianping.,Yao, Biyun.,Yuan, Zhun.,...&Zhou, Zongcan.(2010).Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells.CHEMICO-BIOLOGICAL INTERACTIONS,186(2),166-173.
MLA Zhao, Peng,et al."Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells".CHEMICO-BIOLOGICAL INTERACTIONS 186.2(2010):166-173.
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