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IR@PKUHSC  > 北京大学公共卫生学院  > 毒理学系  > 期刊论文
学科主题: 公共卫生
题名:
Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells
作者: Zhao, Peng1; Zhong, Weijian2; Ying, Xianping2; Yao, Biyun1; Yuan, Zhun1; Fu, Juanling1; Zhou, Zongcan1
关键词: Anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide ; (anti-BPDE) ; Anti-BPDE-transformed cells ; Q-TOF MS/MS ; PCNA ; TPD52
刊名: CHEMICO-BIOLOGICAL INTERACTIONS
发表日期: 2010-07-30
DOI: 10.1016/j.cbi.2010.04.015
卷: 186, 期:2, 页:166-173
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: POLYCYCLIC AROMATIC-HYDROCARBONS ; HUMAN PROSTATE-CANCER ; TUMOR PROTEIN D52 ; GENE-EXPRESSION ; NUCLEAR ANTIGEN ; LUNG-CANCER ; PCNA ; CARCINOMA ; APOPTOSIS ; CYCLE
英文摘要:

In the present study, we investigated the proteomic profiling of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (anti-BPDE)-transformed human bronchial epithelial cell line (16HBE-C) and its parental cell line (16HBE) G0/G1 cells. Differential analysis of proteomic profiling indicated that 67 polypeptides were down-regulated and 77 polypeptides were up-regulated in 16HBE-CG0/G1 cells compared to 16HBE G0/G1 cells. Then 16 differentially expressed protein spots were analyzed with Q-TOF MS/MS. Of these spots, 3 down-regulated polypeptides were identified as sorcin, small ubiquitin-related modifier 2 precursor and eukaryotic translation initiation factor 5A-1, and 9 up-regulated polypeptides were identified as calmodulin, myosin light polypeptide 6, eukaryotic translation initiation factor 6. proliferating cell nuclear antigen (PCNA), tumor protein D52 (TPD52), superoxide dismutase [Cu-Zn], prohibitin, nuclear protein Hcc-1 and vimentin. These proteins are involved in cell proliferation, protein synthesis, signal transduction and carcinogenesis. Western blotting analysis verified the increased expression levels of PCNA and TPD52 in 16HBE-C G0/G1 cells. Based on the clues from proteomic analysis, the migration and invasion capabilities of 16HBE-C and 16HBE cells were tested. The results indicated that 16HBE-C cells showed much higher migration and invasion capabilities than 16HBE cells, and moreover, the suppression of TPD52 by RNAi resulted in significant decrease of migration and invasion capabilities of 16HBE-C cells. These results will be valuable for further investigating and understanding the mechanisms underlying BaP-induced carcinogenesis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30471470 ; 30671781 ; 2002CB512906 ; BMU20090460
项目资助者: National Nature Science Foundation of China ; China National Key Basic Research and Development Program ; Science Foundation of Peking University Health Science Center
WOS记录号: WOS:000279644900009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58451
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China
2.Shanghai Municipal Ctr Dis Control & Prevent, Dept Toxicol, Shanghai 200336, Peoples R China

Recommended Citation:
Zhao, Peng,Zhong, Weijian,Ying, Xianping,et al. Comparative proteomic analysis of anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-transformed and normal human bronchial epithelial G0/G1 cells[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2010,186(2):166-173.
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