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学科主题: 临床医学
题名:
Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver
作者: Li, JX; Li, ZQ; Pang, YZ; Tang, CS
关键词: nuclear nucleoside triphosphatase ; mRNA export ; phosphatidylcholine ; hepatocyte ; phospholipase A(2)
刊名: LIFE SCIENCES
发表日期: 2003-07-11
DOI: 10.1016/S0024-3205(03)00357-6
卷: 73, 期:8, 页:969-980
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: NUCLEOCYTOPLASMIC TRANSPORT ; MEMBRANE CHOLESTEROL ; LIPID-COMPOSITION ; ENVELOPE ; PURIFICATION ; OXIDATION ; ISCHEMIA ; FLUIDITY ; PROTEIN ; ATPASE
英文摘要:

The present study is undertaken to investigate whether the phospholipase A(2) (PLA(2)) influences mRNA nucleocytoplasmic transport evaluated by nucleoside triphosphatase (NTPase) activity and mRNA export in isolated hepatic nuclear envelope. Isolated hepatic nuclei from rat liver were exposed to PLA(2) (10(-5) similar to 10(-2)/ml) with or without incorporation of nuclei with phosphatidylcholine (PC) liposome. Messenger RNA exports and NTPase activities of nuclear membrane were assayed using ATP and GTP as substrates. We found that the RNA efflux, evaluated by [3 H] uridine, was potently decreased in a concentration-dependent manner, by incubation of hepatic nuclei with PLA2, regardless using ATP or GTP as substrates. The PC content in nuclear membrane was also decreased by PLA(2)-treatment. The PC was incorporated into the nuclear membrane by addition of phospholipid liposomes into the incubation mixture. PC incorporation into the nuclear membrane did not alter mRNA export. However this resulted in a significant increase in mRNA export rate in PLA(2)-treated group. Messenger RNA export rate in PLA(2) (10(-3) unit/mL)-treated nuclear membrane was positively correlated with level of PC incorporation, both using ATP and GTP as substrates. The activity of nucleoside triphosphatase, a nuclear membrane-associated enzyme, showed parallel variations with mRNA transport. It is concluded that nuclear PLA(2) plays a regulatory role in RNA transport, which can be antagonized by exogenous PC. These might be pathophysiologically significance, although the mechanisms by which this effect takes place remain to be clarified. (C) 2003 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000183664600002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58459
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China

Recommended Citation:
Li, JX,Li, ZQ,Pang, YZ,et al. Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver[J]. LIFE SCIENCES,2003,73(8):969-980.
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