IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题临床医学
Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver
Li, JX; Li, ZQ; Pang, YZ; Tang, CS
关键词nuclear nucleoside triphosphatase mRNA export phosphatidylcholine hepatocyte phospholipase A(2)
刊名LIFE SCIENCES
2003-07-11
DOI10.1016/S0024-3205(03)00357-6
73期:8页:969-980
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]NUCLEOCYTOPLASMIC TRANSPORT ; MEMBRANE CHOLESTEROL ; LIPID-COMPOSITION ; ENVELOPE ; PURIFICATION ; OXIDATION ; ISCHEMIA ; FLUIDITY ; PROTEIN ; ATPASE
英文摘要

The present study is undertaken to investigate whether the phospholipase A(2) (PLA(2)) influences mRNA nucleocytoplasmic transport evaluated by nucleoside triphosphatase (NTPase) activity and mRNA export in isolated hepatic nuclear envelope. Isolated hepatic nuclei from rat liver were exposed to PLA(2) (10(-5) similar to 10(-2)/ml) with or without incorporation of nuclei with phosphatidylcholine (PC) liposome. Messenger RNA exports and NTPase activities of nuclear membrane were assayed using ATP and GTP as substrates. We found that the RNA efflux, evaluated by [3 H] uridine, was potently decreased in a concentration-dependent manner, by incubation of hepatic nuclei with PLA2, regardless using ATP or GTP as substrates. The PC content in nuclear membrane was also decreased by PLA(2)-treatment. The PC was incorporated into the nuclear membrane by addition of phospholipid liposomes into the incubation mixture. PC incorporation into the nuclear membrane did not alter mRNA export. However this resulted in a significant increase in mRNA export rate in PLA(2)-treated group. Messenger RNA export rate in PLA(2) (10(-3) unit/mL)-treated nuclear membrane was positively correlated with level of PC incorporation, both using ATP and GTP as substrates. The activity of nucleoside triphosphatase, a nuclear membrane-associated enzyme, showed parallel variations with mRNA transport. It is concluded that nuclear PLA(2) plays a regulatory role in RNA transport, which can be antagonized by exogenous PC. These might be pathophysiologically significance, although the mechanisms by which this effect takes place remain to be clarified. (C) 2003 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000183664600002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58459
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Ctr Hlth Sci, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Li, JX,Li, ZQ,Pang, YZ,et al. Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver[J]. LIFE SCIENCES,2003,73(8):969-980.
APA Li, JX,Li, ZQ,Pang, YZ,&Tang, CS.(2003).Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver.LIFE SCIENCES,73(8),969-980.
MLA Li, JX,et al."Phospholipase A(2) inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver".LIFE SCIENCES 73.8(2003):969-980.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, JX]的文章
[Li, ZQ]的文章
[Pang, YZ]的文章
百度学术
百度学术中相似的文章
[Li, JX]的文章
[Li, ZQ]的文章
[Pang, YZ]的文章
必应学术
必应学术中相似的文章
[Li, JX]的文章
[Li, ZQ]的文章
[Pang, YZ]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。