学科主题基础医学
Expression of Fas, p53 and AFP in development of human fetal germ cells in vitro
Wu, J; Chen, Y; Li, T
关键词AFP development in vitro Fas fetal germ cells p53
刊名ZYGOTE
2002-11-01
DOI10.1017/S0967199402004070
10期:4页:333-340
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Developmental Biology ; Reproductive Biology
研究领域[WOS]Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]PORCINE GRANULOSA-CELLS ; ALPHA-FETOPROTEIN GENE ; GLUCOCORTICOID-INDUCED APOPTOSIS ; MONOLAYER-CULTURE ; MEDIATED APOPTOSIS ; HUMAN OVARY ; T-CELL ; MOUSE ; GROWTH ; REPRESSION
英文摘要

In the present study we employed a two-step culture system to study the expression of Fas, p53 and alpha-fetoprotein (AFP) in the development in vitro of human fetal germ cells. p53 mRNA was determined by Northern blotting, and Fas content was assessed by western blotting. RT-nested polymerase chain reaction (RT-nPCR) analysis was performed to determine the expression of AFP mRNA in different stages of fetal follicular development. Follicular cell apoptosis was evaluated by DNA fragmentation analyses (DNA ladder). The results showed that by day 7 of culture approximately one-sixth of fetal germ cells grew to class C oocytes (primary oocytes) from class B oocytes (primordial oocytes) or class A oocytes. On day 45 of culture, one-third of these primary follicles doubled in size. In the meantime, there was a high proportion apoptosis of follicular cells on days 35 or 45 of culture, as evident by a clear ladder pattern of DNA fragmentation upon electrophoretic analysis. Expression of Fas antigen and p53 mRNA increased in a time-dependent manner, while AFP mRNA was expressed on days 10 to 35, and disappeared on day 45. These results indicate that human fetal germ cells can develop in a two-step culture system and AFP may play an active role in the proliferation of these germ cells. At the late stage of follicular development in vitro a number of follicular cells became apoptotic. Moreover, apoptosis may be the mechanism responsible for fetal germ cell regression and the Fas antigen and/or p53-mediate death pathway may be central in the induction of germ cell regression.

语种英语
WOS记录号WOS:000179227800007
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58462
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Beijing Med Univ, Med Coll 3, Dept Obstet & Gynecol, Beijing 100083, Peoples R China
2.Chenzhou 1st Hosp, Div Obstet & Gynecol, Chenzhou 42300, Hunan Province, Peoples R China
3.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
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GB/T 7714
Wu, J,Chen, Y,Li, T. Expression of Fas, p53 and AFP in development of human fetal germ cells in vitro[J]. ZYGOTE,2002,10(4):333-340.
APA Wu, J,Chen, Y,&Li, T.(2002).Expression of Fas, p53 and AFP in development of human fetal germ cells in vitro.ZYGOTE,10(4),333-340.
MLA Wu, J,et al."Expression of Fas, p53 and AFP in development of human fetal germ cells in vitro".ZYGOTE 10.4(2002):333-340.
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