北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR
作者: Zhang, LJ; Wang, MY; Li, RY; Calderone, R
关键词: Aspergillus fumigatus ; expression ; virulence-related genes
刊名: MYCOPATHOLOGIA
发表日期: 2005-10-01
DOI: 10.1007/s11046-005-0141-z
卷: 160, 期:3, 页:201-206
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Mycology
研究领域[WOS]: Mycology
关键词[WOS]: INVASIVE ASPERGILLOSIS ; IDENTIFICATION ; BIOSYNTHESIS ; EPIDEMIOLOGY ; INFECTIONS ; DELETION ; MODEL ; PKSP
英文摘要:

We have measured the transcription of several genes that have been implicated as virulence factors in Aspergillus fumigatus, including fos-1, a histidine kinase, two-component signal protein, rhbA, a ras-related protein required for signaling, pksP, a polyketide synthase involved in biosynthesis of melanin, pabA synthetase, an enzyme catalyzing a late step in the biosynthesis of folate, lysF, a homoconitase related to lysine biosynthesis, and cpcA, the transcriptional activator of the cross-pathway control system of amino acid biosynthesis. Transcription levels were determined from in vitro grown organism as well as from lung tissue from mice infected with A. fumigatus. Our data indicate that fos-1 and rhbA transcription increased significantly during the infection in mice, compared to the other genes whose transcription remained the same (pksP, cpcA, pabA) or decreased slightly (lysF). In vitro measurements of transcription compared to transcription in infected lung tissue demonstrated low levels of fos-1 and rhbA, 20-40-fold increases (cpcA, lysF, pabA), while pksP was not detected from cultures. Our data demonstrate that transcription of these genes differs in vitro versus during disease.

语种: 英语
WOS记录号: WOS:000234426700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58465
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Res Ctr Med Mycol, Dept Dermatol, Beijing, Peoples R China
2.Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20057 USA

Recommended Citation:
Zhang, LJ,Wang, MY,Li, RY,et al. Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR[J]. MYCOPATHOLOGIA,2005,160(3):201-206.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, LJ]'s Articles
[Wang, MY]'s Articles
[Li, RY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, LJ]‘s Articles
[Wang, MY]‘s Articles
[Li, RY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace