IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR
Zhang, LJ; Wang, MY; Li, RY; Calderone, R
关键词Aspergillus fumigatus expression virulence-related genes
刊名MYCOPATHOLOGIA
2005-10-01
DOI10.1007/s11046-005-0141-z
160期:3页:201-206
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]INVASIVE ASPERGILLOSIS ; IDENTIFICATION ; BIOSYNTHESIS ; EPIDEMIOLOGY ; INFECTIONS ; DELETION ; MODEL ; PKSP
英文摘要

We have measured the transcription of several genes that have been implicated as virulence factors in Aspergillus fumigatus, including fos-1, a histidine kinase, two-component signal protein, rhbA, a ras-related protein required for signaling, pksP, a polyketide synthase involved in biosynthesis of melanin, pabA synthetase, an enzyme catalyzing a late step in the biosynthesis of folate, lysF, a homoconitase related to lysine biosynthesis, and cpcA, the transcriptional activator of the cross-pathway control system of amino acid biosynthesis. Transcription levels were determined from in vitro grown organism as well as from lung tissue from mice infected with A. fumigatus. Our data indicate that fos-1 and rhbA transcription increased significantly during the infection in mice, compared to the other genes whose transcription remained the same (pksP, cpcA, pabA) or decreased slightly (lysF). In vitro measurements of transcription compared to transcription in infected lung tissue demonstrated low levels of fos-1 and rhbA, 20-40-fold increases (cpcA, lysF, pabA), while pksP was not detected from cultures. Our data demonstrate that transcription of these genes differs in vitro versus during disease.

语种英语
WOS记录号WOS:000234426700002
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58465
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Hosp 1, Res Ctr Med Mycol, Dept Dermatol, Beijing, Peoples R China
2.Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20057 USA
推荐引用方式
GB/T 7714
Zhang, LJ,Wang, MY,Li, RY,et al. Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR[J]. MYCOPATHOLOGIA,2005,160(3):201-206.
APA Zhang, LJ,Wang, MY,Li, RY,&Calderone, R.(2005).Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR.MYCOPATHOLOGIA,160(3),201-206.
MLA Zhang, LJ,et al."Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR".MYCOPATHOLOGIA 160.3(2005):201-206.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, LJ]的文章
[Wang, MY]的文章
[Li, RY]的文章
百度学术
百度学术中相似的文章
[Zhang, LJ]的文章
[Wang, MY]的文章
[Li, RY]的文章
必应学术
必应学术中相似的文章
[Zhang, LJ]的文章
[Wang, MY]的文章
[Li, RY]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。