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Total chemical synthesis of human interferon alpha-2b via native chemical ligation
Li, Jing1,2; Lehmann, Clara1,3; Chen, Xishan1; Romerio, Fabio1; Lu, Wuyuan1
关键词interferon native chemical ligation peptide synthesis synthetic protein
刊名JOURNAL OF PEPTIDE SCIENCE
2015-07-01
DOI10.1002/psc.2760
21期:7页:554-560
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Analytical
资助者National Institutes of Health ; National Institutes of Health
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SYSTEMIC-LUPUS-ERYTHEMATOSUS ; D-PEPTIDE INHIBITORS ; DENDRITIC CELLS ; I INTERFERONS ; MUTATIONAL ANALYSIS ; IMMUNE-COMPLEXES ; HIV-1 ENTRY ; IFN-ALPHA ; PROTEINS ; INDUCTION
英文摘要

Interferon-alpha (IFN) is a cytokine that orchestrates innate and adaptive immune responses and potently inhibits proliferation of normal and tumor cells. These properties have warranted the use of IFN in clinical practice for the treatment of several viral infections and malignancies. However, overexpression of IFN leads to immunopathology observed in the context of chronic viral infections and autoimmune conditions. Thus, it is desirable to develop therapeutic approaches that aim at suppressing excessive IFN production. To that end, artificial evolution of peptides from phage display libraries represents a strategy that seeks to disrupt the interaction between IFN and its cell surface receptor and thus inhibit the ensuing biological effects. Mirror-image phage display that screens peptide libraries against the D-enantiomer is particularly attractive because it allows for identification of proteolysis-resistant D-peptide inhibitors. This approach, however, relies on the availability of chemically synthesized D-IFN composed entirely of D-amino acids. Here, we describe the synthesis and biological properties of IFN2b of 165 amino acid residues produced by native chemical ligation, which represents an important first step toward the discovery of D-peptide antagonists with potential therapeutic applications. Copyright (c) 2015 European Peptide Society and John Wiley & Sons, Ltd.

语种英语
所属项目编号AI072732 ; AI061482
资助者National Institutes of Health ; National Institutes of Health
WOS记录号WOS:000356813900006
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58498
Collection北京大学公共卫生学院
作者单位1.Univ Cologne, Dept Internal Med 1, D-50931 Cologne, Germany
2.Univ Maryland, Sch Med, Inst Human Virol, Baltimore, MD 21201 USA
3.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Epidemiol & Biostatist, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Li, Jing,Lehmann, Clara,Chen, Xishan,et al. Total chemical synthesis of human interferon alpha-2b via native chemical ligation[J]. JOURNAL OF PEPTIDE SCIENCE,2015,21(7):554-560.
APA Li, Jing,Lehmann, Clara,Chen, Xishan,Romerio, Fabio,&Lu, Wuyuan.(2015).Total chemical synthesis of human interferon alpha-2b via native chemical ligation.JOURNAL OF PEPTIDE SCIENCE,21(7),554-560.
MLA Li, Jing,et al."Total chemical synthesis of human interferon alpha-2b via native chemical ligation".JOURNAL OF PEPTIDE SCIENCE 21.7(2015):554-560.
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