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学科主题: 临床医学
题名:
Validation of glaucoma-like features in the rat episcleral vein cauterization model
作者: Bai Yujing1,2; Zhu Yingting2; Chen Qin3; Xu Jing4; Sarunic, Marinko V.4; Saragovi, Uri H.5,6; Zhuo Yehong2
关键词: glaucoma ; experimental animal model ; retinal neurons ; rat
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-01-20
DOI: 10.3760/cma.j.issn.0366-6999.20132398
卷: 127, 期:2, 页:359-364
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CHRONIC OCULAR HYPERTENSION ; NECROSIS-FACTOR-ALPHA ; GANGLION-CELL DEATH ; OPEN-ANGLE GLAUCOMA ; OPTIC-NERVE DAMAGE ; IN-VIVO ; MOUSE MODEL ; MUTATED MOUSE ; IRIS ATROPHY ; DBA/2J MICE
英文摘要:

Background Glaucoma, an irreversible optic nerve neuropathy, always results in blindness. This study aimed to evaluate glaucoma-like features in the rat episcleral vein cauterization (EVC) model by multiple in vivo and in vitro evidences.

Methods Wistar rat was used in this study. The elevated intraocular pressure (IOP) was induced by cauterization of three episcleral veins. IOP was monitored with Tono-Pen XL tonometer. Time-dependent changes to the neuronal retinal layers were quantified by Fourier domain-optical coherence tomography. The function of retina was evaluated by electroretinogram (ERG). Survival of retinal ganglion cells (RGCs) was quantified by retrograde labeling. Histology study was performed with retinal sections stained with hematoxylin-eosin, glial fibrillary acidic protein, and neuronal nuclear antigen. Retina and aqueous humor protein were extracted and cytotoxic protein tumor necrosis factor alpha (TNF-alpha) and alpha-2 macroglobulin (alpha 2m) were measured with Western blotting.

Results EVC is a relatively facile intervention, with low failure rates (<5%). After surgical intervention, chronic mild IOP elevation (about 1.6-fold over normal, P <0.05) was induced for at least 6 weeks without requiring a second intervention. High IOP causes chronic and progressive loss of RGCs (averaging about 4% per week), progressive thinning of neuronal retinal layers (3-5 mu m per week), and reduction of a- and b-wave in ERG. EVC method can also induce glial cell activation and alterations of inflammation proteins, such as TNF-alpha and alpha 2m.

Conclusion EVC method can establish a robust, reliable, economic and highly reproducible glaucomatous animal model.

语种: 英语
所属项目编号: 10251008901000028
项目资助者: Guangdong Province Universities ; Colleges Pearl River Scholar Funded Scheme ; Guangdong Province Natural Science Foundation
WOS记录号: WOS:000330606900026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58506
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Ophthalmol, Key Lab Vis Loss & Restorat, Minist Educ, Beijing 100044, Peoples R China
2.Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China
3.Nanjing Med Univ, Affiliated Hosp 1, Dept Ophthalmol, Nanjing 210029, Jiangsu, Peoples R China
4.Simon Fraser Univ, Burnaby, BC V5A 1S6, Canada
5.McGill Univ, Jewish Gen Hosp, Bloomfield Ctr Res Aging, Lady Davis Inst,Dept Oncol,Dept Pharmacol & Thera, Montreal, PQ H3T 1E2, Canada
6.McGill Univ, Jewish Gen Hosp, Bloomfield Ctr Res Aging, Ctr Canc,Lady Davis Inst, Montreal, PQ H3T 1E2, Canada

Recommended Citation:
Bai Yujing,Zhu Yingting,Chen Qin,et al. Validation of glaucoma-like features in the rat episcleral vein cauterization model[J]. CHINESE MEDICAL JOURNAL,2014,127(2):359-364.
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