IR@PKUHSC  > 北京大学第三临床医学院  > 皮肤科
学科主题临床医学
Metallothionein-null mice exhibit reduced tolerance to ultraviolet B injury in vivo
Wang, WH; Li, LF; Zhang, BX; Lu, XY
刊名CLINICAL AND EXPERIMENTAL DERMATOLOGY
2004
29期:1页:57-61
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]MOUSE SKIN ; UVB INJURY ; KERATINOCYTES ; DAMAGE
英文摘要

Events that induce expression of the metallothionein (MT) gene, such as injection of cadmium chloride, cold stress or topical application of 1,25-dihydroxyvitamin D-3, can deplete the number of ultraviolet (UV) B-induced sunburn cells (SBC) in mouse skin in vivo. MT-null mouse skin explants exhibit reduced tolerance to UVB injury in vitro. However, the in vivo response of MT-null mice to UVB injury has not been investigated. In the present study, we investigated the role of the MT gene on UVB injury in vivo. MT-null mice that are deficient in MT-I and MT-II genes were studied and compared with homozygous wild-type mice. Mouse dorsal skin was irradiated with 0.05, 0.70 and 1.40 J/cm(2) UVB. The thickness of the dorsal skin was measured with a spring micrometer before and 24 h after UVB irradiation. In addition, SBC were counted 24 h after UVB irradiation. No significant difference was found in the change of skin thickness between MT-null mice and control mice irradiated with low-dose UVB (0.05 J/cm(2)) (Student′s t-test, t = 1.519, P = 0.167). At higher doses (0.70 and 1.40 J/cm(2)), the skin of MT-null mice became much thicker than that of control mice (Student′s t-test, t = 6.576, P < 0.01 and t = 3.142, P = 0.007, respectively). More SBC were detected in MT-null mice skin irradiated with the highest dose of UVB (1.40 J/cm(2)) (Student′s t-test, t = 4.258, P < 0.01). These results suggest that the MT gene in mice has a photoprotective role in vivo.

语种英语
WOS记录号WOS:000188127900015
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58507
Collection北京大学第三临床医学院_皮肤科
作者单位1.Peking Univ, Dept Toxicol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Dept Dermatol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Wang, WH,Li, LF,Zhang, BX,et al. Metallothionein-null mice exhibit reduced tolerance to ultraviolet B injury in vivo[J]. CLINICAL AND EXPERIMENTAL DERMATOLOGY,2004,29(1):57-61.
APA Wang, WH,Li, LF,Zhang, BX,&Lu, XY.(2004).Metallothionein-null mice exhibit reduced tolerance to ultraviolet B injury in vivo.CLINICAL AND EXPERIMENTAL DERMATOLOGY,29(1),57-61.
MLA Wang, WH,et al."Metallothionein-null mice exhibit reduced tolerance to ultraviolet B injury in vivo".CLINICAL AND EXPERIMENTAL DERMATOLOGY 29.1(2004):57-61.
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