IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Role of Vimentin in the Inhibitory Effects of Low-Molecular-Weight Heparin on PC-3M Cell Adhesion to, and Migration through, Endothelium
Pan, Yan; Lei, Tianluo; Teng, Bao; Liu, Jihong; Zhang, Jianzhao; An, Yu; Xiao, Yuan; Han, Jing; Pan, Xueyang; Wang, Junhua; Yu, Heming; Ren, Hong; Li, Xuejun1
刊名JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
2011-10-01
DOI10.1124/jpet.111.182055
339期:1页:82-92
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]IN-VITRO ; TRANSENDOTHELIAL MIGRATION ; CYTOSOLIC CALCIUM ; BREAST-CANCER ; TUMOR-CELLS ; EXPRESSION ; CYTOSKELETON ; CONTACT ; MATRIX ; PERMEABILITY
英文摘要

Low-molecular-weight heparin (LMWH) has been used in cancer patients with venous thromboembolic complications, resulting in a higher survival rate and an inhibitory action on experimental metastasis. In the present study, human umbilical vein endothelial cells (HUVECs) were treated with LMWH for 24 h. We found that the resulting HUVECs could significantly inhibit the highly metastatic human prostate cancer cell line (PC-3M) in terms of its adhesion to the endothelium and migration across the endothelium, according to scanning electron microscopy. We also determined the elevated levels of endothelial intercellular Ca2+ concentration after the adhesion of PC-3M cells to HUVECs was greatly reduced by incubation with LMWH. Using proteomics, we surveyed the global protein changes in HUVECs after LMWH treatment and identified four down-regulated proteins that were possible isoforms of cytoskeletal vimentin intermediate filaments, cartilage-derived C-type lectin, and serine/threonine protein phosphatase 1 beta (PP-1B). LMWH affected the morphology of vimentin and the expression levels of alpha(v) integrin and PP-1B in HUVECs bound to PC-3M cells. Vimentin assists in the adhesion of PC-3M cells, which was confirmed by short interfering RNA experiments. Furthermore, the direct binding of purified vimentin protein with LMWH was detected with surface plasmon resonance methods. However, when we used fluorescence-labeled heparin for 24 h to identify whether this binding occurred within cells, heparin was distributed principally around endothelial cells. Taken together, these findings suggest that the monoincubation of LMWH with HUVECs could inhibit PC-3M cell adhesion to, and migration through, endothelium. LMWH′s regulation of vimentin plays a role in the antimetastatic action.

语种英语
WOS记录号WOS:000294998900009
项目编号81020108031 ; 30572202 ; 30973558 ; 30772571 ; 30901803 ; 30901815 ; 2009ZX09103-144 ; B07001
资助机构National Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58523
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Peking Univ, Inst Syst Biomed, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
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Pan, Yan,Lei, Tianluo,Teng, Bao,et al. Role of Vimentin in the Inhibitory Effects of Low-Molecular-Weight Heparin on PC-3M Cell Adhesion to, and Migration through, Endothelium[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2011,339(1):82-92.
APA Pan, Yan.,Lei, Tianluo.,Teng, Bao.,Liu, Jihong.,Zhang, Jianzhao.,...&Li, Xuejun.(2011).Role of Vimentin in the Inhibitory Effects of Low-Molecular-Weight Heparin on PC-3M Cell Adhesion to, and Migration through, Endothelium.JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,339(1),82-92.
MLA Pan, Yan,et al."Role of Vimentin in the Inhibitory Effects of Low-Molecular-Weight Heparin on PC-3M Cell Adhesion to, and Migration through, Endothelium".JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 339.1(2011):82-92.
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