IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Expression of NASG gene and its role in human nasopharyngeal homogenous tissue cells
Liu, ZQ; Tian, YQ; Peng, C; Hu, YF; Zhou, M; Ouyang, J; Li, XL; Liu, HY; Zhang, BC; Li, GY
关键词nasopharyngeal carcinoma NASG RNA, antisense
刊名CHINESE MEDICAL JOURNAL
2005-07-05
118期:13页:1076-1080
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]ULTRAVIOLET-RADIATION FRACTIONATION ; POLYMERASE CHAIN-REACTION ; CDNA MICROARRAY ; CARCINOMA ; IDENTIFICATION ; AMPLIFICATION ; FAMILY ; RNA
英文摘要

Background The NASG gene has been confirmed as a tumor-suppressor gene candidate related to nasopharyngeal carcinoma (NPC) by previous studies. We further investigated the expression and the role of NASG in the homogeneous tissue cells by microdissecting the samples of tissue from human NPC, and introduced a new way to study the expression of specific genes in tumor tissue.

Methods The RNAlater reagent was used to preserve the samples of tissue from the nasopharynx of NPC patients. The samples were microdissected to harvest the homogeneous tissue cells and then total RNA was isolated from them. The antisense RNA ( aRNA) was amplified from the total RNA by "in vitro" transcription (IVT) ". We investigated NASG expression in the homogeneous tumor cells of NPC (22 samples) and compared it with that in the pure epithelial pillar cells of normal nasopharyngeal ( 10 samples) by semi-quantitative reverse transcription-polymerase chain reaction (sqRT-PCR).

Results The high quality total RNA could be harvested from the microdissected homogeneous tissue cells of the nasopharynx, then sufficient aRNA was derived from it. NASG gene expression was identified using aRNA by sqRT-PCR and showed that there was significant difference between the average value of case groups and that of control group (t = - 5. 275, df = 30, P < 0.001). The NASG gene in the subgroups WHOII tended to express lower levels than those in the subgroup WHO III although this difference was not statistically siginificant ( t 1.5 84, df = 20, P = 0. 129 > 0. 05).

Conclusions Microdissection was an effective method to obtain the homogeneous tissue cells of nasopharyngeal tissue (including the samples of NPC and non-NPC) in our study. Sufficient aRNA from amplifiying total RNA could be used in sqRT-PCR to analyse the expression of NASG in the pure tissue cells. NASG should be a tumor-suppression gene candidate regarding to NPC.

语种英语
WOS记录号WOS:000230474300004
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58528
专题北京大学第三临床医学院
作者单位1.Cent S Univ, Xiangya Hosp, Dept Otolaryngol, Changsha 410078, Peoples R China
2.Peking Univ, Hosp 3, Dept Ent, Beijing 100083, Peoples R China
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Liu, ZQ,Tian, YQ,Peng, C,et al. Expression of NASG gene and its role in human nasopharyngeal homogenous tissue cells[J]. CHINESE MEDICAL JOURNAL,2005,118(13):1076-1080.
APA Liu, ZQ.,Tian, YQ.,Peng, C.,Hu, YF.,Zhou, M.,...&Li, GY.(2005).Expression of NASG gene and its role in human nasopharyngeal homogenous tissue cells.CHINESE MEDICAL JOURNAL,118(13),1076-1080.
MLA Liu, ZQ,et al."Expression of NASG gene and its role in human nasopharyngeal homogenous tissue cells".CHINESE MEDICAL JOURNAL 118.13(2005):1076-1080.
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